典型文献
Metabolic responses of indigenous bacteria in chicken faeces and maggots to multiple antibiotics via heavy water labeled single-cell Raman spectroscopy
文献摘要：
The use of maggots derived from chicken faeces as fish diets might serve as a vehicle for the widespread of multiple antibiotic resistant bacteria (ARB) in the environment.Heavy water labeled single-cell Raman spectroscopy (D2O-Raman) was applied to detect the metabolic responses of indigenous bacteria in chicken faeces and maggots to different concentrations of combined colistin,kanamycin,and vancomycin.By incubating the samples with D2O and antibiotics,metabolically active bacterial cells to antibiotics were distinguished from those inactive by the exhibition of C-D Raman band.Using the C-D band as a universal metabolic biomarker,96％ and 100％ of cells in chicken faeces and maggots were revealed to be metabolically active to 1 x minimum inhibition concentration (MIC) of the afore-mentioned antibiotics.A noticeable decrease in the percentage of active cells from 96％ to 76％ in faeces and 100％ to 93％ in maggots was observed at 5 x MIC of antibiotics.However,these ratios were still far above that obtained from the same faeces (1.84％) and maggots(0.51％) samples using a cultivation method,indicating the wide presence of nongrowing but metabolically active bacterial cells under antibiotic treatment.Conclusively,the culture-independent D2O-Raman approach detected and quantified a large portion of metabolically active indigenous bacteria to multiple antibiotics in their native environments,illustrating the great potential risks of these active cells to spread antibiotic resistance via food chain.
文献关键词：
中图分类号：
作者姓名：
Oladipo Oladiti Olaniyi;Hongzhe Li;Yongguan Zhu;Li Cui
作者机构：
Key Lab of Urban Environment and Health,Institute of Urban Environment,Chinese Academy of Sciences,Xiamen 361021,China;Department of Microbiology,Federal University of Technology,PMB 704 Akure,Nigeria;University of Chinese Academy of Sciences,Beijing 100049,China
文献出处：
引用格式：
[1]Oladipo Oladiti Olaniyi;Hongzhe Li;Yongguan Zhu;Li Cui-.Metabolic responses of indigenous bacteria in chicken faeces and maggots to multiple antibiotics via heavy water labeled single-cell Raman spectroscopy)[J].环境科学学报（英文版）,2022(03):394-402
A类：
maggots,afore,nongrowing
B类：
Metabolic,responses,indigenous,chicken,faeces,multiple,antibiotics,via,heavy,water,labeled,single,Raman,spectroscopy,use,derived,from,fish,diets,might,vehicle,widespread,resistant,ARB,Heavy,D2O,was,applied,different,concentrations,combined,colistin,kanamycin,vancomycin,By,incubating,samples,metabolically,bacterial,cells,were,distinguished,those,inactive,by,exhibition,band,Using,universal,biomarker,revealed,minimum,inhibition,MIC,mentioned,noticeable,decrease,percentage,observed,However,these,ratios,still,far,above,that,obtained,same,using,cultivation,method,indicating,presence,but,under,treatment,Conclusively,culture,independent,approach,detected,quantified,large,portion,their,native,environments,illustrating,great,potential,risks,resistance,food,chain
AB值：
0.451266
相似文献
机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
