Pig manure,rich in antibiotics and metals,is widely applied in paddy fields as a soil conditioner,triggering the proliferation of antibiotic resistance genes(ARGs)in soil.However,comprehensive studies on the effects of manure fertilization on the abundance of ARGs and their influencing factors are still insufficient.Here,pig manure and manure-amended and inorganic-amended soils were collected from 11 rice-cropping regions in eastern China,and the accumulation of antibiotics,metals,and ARGs was assessed simultaneously.The results showed that manure fertilization led to antibiotic residues and increased the metal content(i.e.,Zn,Cu,Ni,and Cr).Tetracycline and sulfonamide resistance genes(tetM,tetO,sul1,and sul2)were also significantly enhanced with manure fertilization.According to variance partitioning analysis,the most important factors that individually influenced ARGs were soil physicochemical properties,accounting for 12％of the variation.Significant correlations between soil nutrients and ARGs indicated that manure application enhanced the growth of resistant microorganisms by supplying more nutrients.Metals and antibiotics contributed 9％and 5％to the variations in ARGs,respectively.Their co-occurrence also increased the enrichment of ARGs,as their interactions accounted for 2％of the variation in ARGs.Interestingly,Cu was significantly related to most ARGs in the soil(r=0.26-0.52,p＜0.05).Sulfapyridine was significantly related to sul2,and tetracycline resistance genes were positively related to doxycycline.This study highlighted the risks of antibiotic and ARG accumulation with manure fertilization and shed light on the essential influencing factors of ARGs in paddy soils.

Yuwei Guo;Xian Xiao;Yuan Zhao;Jianguo Liu;Jizhong Zhou;Bo Sun;Yuting Liang

School of Environmental and Safety Engineering,Changzhou University,Changzhou 213164,China;State Key Laboratory of Soil and Sustainable Agriculture,Institute of Soil Science,Chinese Academy of Sciences,Nanjing 210008,China;Institute for Environmental Genomics,Department of Microbiology and Plant Biology,and School of Civil Engineering and Environmental Sciences,University of Oklahoma,Norman,OK 73019,USA;State Key Joint Laboratory of Environment Simulation and Pollution Control,School of Environment,Tsinghua University,Beijing 100084,China;Earth and Environmental Sciences,Lawrence Berkeley National Laboratory,Berkeley,CA 94270,USA

环境科学与工程前沿

[1]Yuwei Guo;Xian Xiao;Yuan Zhao;Jianguo Liu;Jizhong Zhou;Bo Sun;Yuting Liang-.Antibiotic resistance genes in manure-amended paddy soils across eastern China:Occurrence and influencing factors)[J].环境科学与工程前沿,2022(07):96-106

Sulfapyridine

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