典型文献
Antibacterial and antibiofilm activity of peptide PvGBP2 against pathogenic bacteria that contaminate Auricularia auricular culture bags
文献摘要：
Bacteria contamination in Auricularia auricular culture bags reduces yield and increases the risk of food safety. In this study, 5 species of bacteria, mainly gram-positive bacteria including three species of Bacillus spp., Arthrobacter arilaitensis and Staphylococcus warneri, were isolated and identified from bacteria-contaminated A. auricular culture bags. An in silico predicted antimicrobial peptide from the β-1,3-glucan-binding protein sequence of Penaeus vannamei, designated PvGBP2 (FLKLGRKSRYGMLKL), was screened and its antibacterial effect and mechanism of action on the isolated Bacillus spp. explored. The minimal inhibitory concentrations (MIC) of PvGBP2 on Bacillus spp. were 15.6–31.25 μg/mL. Peptide PvGBP2 could inhibit Bacillus subtilis in A. auricular culture bags to maintain growth and yield of A. auricular. Transmission electron microscopy (TEM) revealed that PvGBP2 kills bacteria by perforating the cell wall, destroying membrane integrity and resulting in the leakage of intracellular solutes. In addition, PvGBP2 inhibits biofilm formation by B. subtilis by 90.6％ at 1 × MIC. Thus, peptide PvGBP2 could be potentially applied as an antibacterial agent to control bacterial infection of A. auricular cultivation and the spread of foodborne pathogens.
文献关键词：
中图分类号：
作者姓名：
Shen Yang;Zijin Yuan;Jude Juventus Aweya;Shanggui Deng;Wuyin Weng;Yueling Zhang;Guangming Liu
作者机构：
College of Food and Biological Engineering,Xiamen Key Laboratory of Marine Functional Food,Jimei University,Xiamen 361021,China;Department of Biology,Guangdong Provincial Key Laboratory of Marine Biotechnology,Shantou University,Shantou 515063,China;College of Food and Pharmacy,Zhejiang Ocean University,Zhoushan 316000,China
文献出处：
引用格式：
[1]Shen Yang;Zijin Yuan;Jude Juventus Aweya;Shanggui Deng;Wuyin Weng;Yueling Zhang;Guangming Liu-.Antibacterial and antibiofilm activity of peptide PvGBP2 against pathogenic bacteria that contaminate Auricularia auricular culture bags)[J].食品科学与人类健康（英文）,2022(06):1607-1613
A类：
PvGBP2,arilaitensis,FLKLGRKSRYGMLKL,perforating
B类：
Antibacterial,antibiofilm,activity,peptide,against,pathogenic,that,Auricularia,auricular,culture,bags,Bacteria,contamination,reduces,yield,increases,risk,safety,In,this,study,species,mainly,gram,positive,including,three,Bacillus,spp,Arthrobacter,Staphylococcus,warneri,were,isolated,identified,from,contaminated,silico,predicted,antimicrobial,glucan,binding,protein,sequence,Penaeus,vannamei,designated,was,screened,antibacterial,effect,mechanism,action,explored,minimal,inhibitory,concentrations,MIC,Peptide,could,subtilis,maintain,growth,Transmission,electron,microscopy,TEM,revealed,kills,by,wall,destroying,membrane,integrity,resulting,leakage,intracellular,solutes,addition,inhibits,formation,Thus,be,potentially,applied,agent,control,infection,cultivation,spread,foodborne,pathogens
AB值：
0.527406
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