典型文献
A comprehensive method to explore inhibitory kinetics and mechanisms of an anticoagulant peptide derived from Crassostrea gigas
文献摘要：
A comprehensive method was applied to evaluate the anticoagulant activity of a novel anticoagulant peptide (NAESLRK) derived from oyster (Crassostrea gigas). The anticoagulant peptide drastically reduced the extrinsic clotting activity and also impaired the intrinsic clotting activity slightly. Consistent with clotting data, the thrombin peak height was reduced to 84.7 nmol/L from 123.4 nmol/L, and thrombin generation time was delayed to 4.67 min from 4.42 min when the extrinsic trigger was applied. The inhibitory kinetics of FXIa, FIXa, FXa, FIIa, and APC in a purified component system rationally explained the reduction of extrinsic clotting activity and impairment of thrombin generation. Besides the inhibition of FXa and FIIa activity, the activation processes of FX and FⅡ by intrinsic/extrinsic tenase complex and prothrombinase were also damaged. The anticoagulant activity in the plasma system was the result of comprehensive inhibition of various factors. The research provided a novel method for anticoagulant evaluation and inhibitory mechanism of bioactive peptides from food products.
文献关键词：
中图分类号：
作者姓名：
Shuzhen Cheng;Di Wu;Hanxiong Liu;Xianbing Xu;Beiwei Zhu;Ming Du
作者机构：
Department of Food Science and Engineering,National Engineering Research Center of Seafood,Dalian Polytechnic University,Dalian 116034,China;Beijing Advanced Innovation Center for Food Nutrition and Human Health,College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China
文献出处：
引用格式：
[1]Shuzhen Cheng;Di Wu;Hanxiong Liu;Xianbing Xu;Beiwei Zhu;Ming Du-.A comprehensive method to explore inhibitory kinetics and mechanisms of an anticoagulant peptide derived from Crassostrea gigas)[J].食品科学与人类健康（英文）,2022(06):1491-1499
A类：
NAESLRK,FXIa,FIXa,FXa,FIIa,tenase,prothrombinase
B类：
comprehensive,method,explore,inhibitory,kinetics,mechanisms,anticoagulant,derived,from,Crassostrea,gigas,was,applied,evaluate,activity,novel,oyster,drastically,reduced,extrinsic,clotting,also,impaired,intrinsic,slightly,Consistent,data,peak,height,nmol,generation,delayed,when,trigger,APC,purified,component,system,rationally,explained,reduction,impairment,Besides,inhibition,activation,processes,by,complex,were,damaged,plasma,result,various,factors,research,provided,evaluation,bioactive,peptides,food,products
AB值：
0.39961
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