典型文献
Diagnostic role of NPY methylation in patients with colorectal cancer
文献摘要：
Objectives: A growing number of studies have shown that methylation biomarkers play an important role in on-cogenesis. This study aimed to explore the diagnostic role of neuropeptide Y (NPY) methylation in colorectal cancer (CRC). Methods: mRNA and protein expression, methylation, survival benefits, and immune cell infiltration were ana-lyzed using bioinformatics tools across all tumors from The Cancer Genome Atlas. NPY methylation in CRC was further validated in CRC tissues, fecal samples, and cell lines. Analyses of NPY methylation were performed using Sequenome EpiTYPER and quantitative PCR. Retrieval of NPY expression in cell lines was tested using real-time PCR and western blotting. Results: Bioinformatic analysis showed that the methylation level of NPY increased in most carcinomas (P<0.05). Moreover, statistical correlations were observed between NPY transcriptional expression and CD4+ T cells, macrophages, and dendritic cells in colon cancer (P<0.05). Similar results were obtained for CD4+ T cells, neutrophils, and NPY in rectal cancer (P<0.05). Our results showed that NPY was hypermethylated in CRC tissues and fecal exfoliated cells (P<0.05). Fecal NPY methylation was observed in 82.5％ sensitive for primary tumors, 46.3％ for intestinal polyps (including adeno-matous, serrated, and inflammatory polyps), and 23.4％ of healthy controls. Overall, fecal NPY methylation was 76.6％specific. For cell lines, in vivo experiments demonstrated that 5-aza-2′-deoxycytidine downregulated the methylation of NPY and restored its mRNA level (P<0.05). Conclusions: This study indicates that NPY is hypermethylated in CRC, and that NPY methylation in fecal DNA is a potential noninvasive diagnostic biomarker for Chinese patients with CRC.
文献关键词：
中图分类号：
作者姓名：
Jing Wang;Yuan He
作者机构：
Department of Pathology,the First Affiliated Hospital of USTC,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei 230001,China;Department of Radiation Oncology,the First Affiliated Hospital of USTC,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei 230001,China;State Key Laboratory of Oncology in South China,Collaborative Innovation Center for Cancer Medicine,Sun Yat-sen University Cancer Cen-ter,Guangzhou 510060,China
文献出处：
引用格式：
[1]Jing Wang;Yuan He-.Diagnostic role of NPY methylation in patients with colorectal cancer)[J].中国科学技术大学学报,2022(06):10-21
A类：
cogenesis,Sequenome,matous
B类：
Diagnostic,role,NPY,methylation,patients,colorectal,cancer,Objectives,growing,number,studies,have,shown,that,biomarkers,play,important,This,study,aimed,explore,diagnostic,neuropeptide,CRC,Methods,protein,expression,survival,benefits,immune,infiltration,were,lyzed,using,bioinformatics,tools,across,tumors,from,Cancer,Genome,Atlas,was,further,validated,tissues,fecal,samples,lines,Analyses,performed,EpiTYPER,quantitative,Retrieval,tested,real,western,blotting,Results,Bioinformatic,analysis,showed,level,increased,most,carcinomas,Moreover,statistical,correlations,observed,between,transcriptional,CD4+,cells,macrophages,dendritic,colon,Similar,results,obtained,neutrophils,Our,hypermethylated,exfoliated,Fecal,sensitive,primary,intestinal,polyps,including,adeno,serrated,inflammatory,healthy,controls,Overall,specific,For,vivo,experiments,demonstrated,aza,deoxycytidine,downregulated,restored,Conclusions,indicates,potential,noninvasive,Chinese
AB值：
0.512725
相似文献
机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
