典型文献
Screening of immune cell activators from Astragali Radix using a comprehensive two-dimensional NK-92MI cell membrane chromatography/C18 column/time-of-flight mass spectrometry system
文献摘要：
Astragali Radix(AR)is a clinically used herbal medicine with multiple immunomodulatory activities that can strengthen the activity and cytotoxicity of natural killer(NK)cells.However,owing to the complexity of its composition,the specific active ingredients in AR that act on NK cells are not clear yet.Cell membrane chromatography(CMC)is mainly used to screen the active ingredients in a complex system of herbal medicines.In this study,a new comprehensive two-dimensional(2D)NK-92MI CMC/C18 column/time-of-flight mass spectrometry(TOFMS)system was established to screen for potential NK cell acti-vators.To obtain a higher column efficiency,3-mercaptopropyltrimethoxysilane-modified silica was synthesized to prepare the NK-92MI CMC column.In total,nine components in AR were screened from this system,which could be washed out from the NK-92MI/CMC column after 10 min,and they showed good affinity for NK-92MI/CMC column.Two representative active compounds of AR,isoastragaloside Ⅰ and astragaloside Ⅳ,promoted the killing effect of NK cells on K562 cells in a dose-dependent manner.It can thus suggest that isoastragaloside Ⅰ and astragaloside Ⅳ are the main immunomodulatory compo-nents of AR.This comprehensive 2D NK-92MI CMC analytical system is a practical method for screening immune cell activators from other herbal medicines with immunomodulatory effects.
文献关键词：
中图分类号：
作者姓名：
Xinyi Chai;Yanqiu Gu;Lei Lv;Chun Chen;Fei Feng;Yan Cao;Yue Liu;Zhenyu Zhu;Zhanying Hong;Yifeng Chai;Xiaofei Chen
作者机构：
School of Pharmacy,Shanghai Key Laboratory for Pharmaceutical Metabolite Research,Naval Medical University(Second Military Medical University),Shanghai 200433,China;Department of Pharmacy,Shanghai Ninth People's Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,201999,China;Department of Pharmacy,Shanghai Eastern Hepatobiliary Surgery Hospital,Naval Medical University(Second Military Medical University),Shanghai,200438,China
文献出处：
引用格式：
[1]Xinyi Chai;Yanqiu Gu;Lei Lv;Chun Chen;Fei Feng;Yan Cao;Yue Liu;Zhenyu Zhu;Zhanying Hong;Yifeng Chai;Xiaofei Chen-.Screening of immune cell activators from Astragali Radix using a comprehensive two-dimensional NK-92MI cell membrane chromatography/C18 column/time-of-flight mass spectrometry system)[J].药物分析学报（英文）,2022(05):725-732
A类：
vators,mercaptopropyltrimethoxysilane,isoastragaloside
B类：
Screening,immune,activators,from,Astragali,Radix,using,comprehensive,two,dimensional,NK,92MI,membrane,chromatography,C18,column,flight,mass,spectrometry,system,clinically,used,herbal,multiple,immunomodulatory,activities,that,can,strengthen,activity,cytotoxicity,natural,killer,cells,However,owing,complexity,its,composition,specific,active,ingredients,not,clear,yet,Cell,CMC,mainly,medicines,In,this,study,new,2D,TOFMS,established,potential,To,obtain,higher,efficiency,modified,silica,synthesized,prepare,total,nine,components,were,screened,which,could,be,washed,out,after,they,showed,good,affinity,Two,representative,compounds,promoted,killing,K562,dose,dependent,manner,It,thus,suggest,This,analytical,practical,method,screening,other,effects
AB值：
0.457515
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