Manipulating and real-time monitoring of neuronal activities with cell-type specificity and precise spatiotemporal resolu-tion during animal behavior are fundamental technologies for exploring the functional connectivity, information transmis-sion, and physiological functions of neural circuits in vivo. However, current techniques for optogenetic stimulation and neuronal activity recording mostly operate independently. Here, we report an all-fiber-transmission photometry system for simultaneous optogenetic manipulation and multi-color recording of neuronal activities and the neurotransmitter release in a freely moving animal. We have designed and manufactured a wavelength-independent multi-branch fiber bundle to enable simultaneous optogenetic manipulation and multi-color recording at different wavelengths. Further, we combine a laser of narrow linewidth with the lock-in amplification method to suppress the optogenetic stimulation-induced artifacts and channel crosstalk. We show that the collection efficiency of our system outperforms a traditional epi-fluorescence system. Further, we demonstrate successful recording of dynamic dopamine (DA) responses to unexpected rewards in the nucleus accumbens (NAc) in a freely moving mouse. We also show simultaneous dual-color recording of neuronal Ca2+ signals and DA dynamics in the NAc upon delivering an unexpected reward and the simultaneous optogenetic activ-ating at dopaminergic terminals in the same location. Thus, our multi-function fiber photometry system provides a com-patible, efficient, and flexible solution for neuroscientists to study neural circuits and neurological diseases.

Zhongyang Qi;Qingchun Guo;Shu Wang;Mingyue Jia;Xinwei Gao;Minmin Luo;Ling Fu

Britton Chance Center for Biomedical Photonics,Wuhan National Laboratory for Optoelectronics,Huazhong University of Science and Technology,Wuhan 430074,China;MoE Key Laboratory for Biomedical Photonics,School of Engineering Sciences,Huazhong University of Science and Technology,Wuhan 430074,China;National Institute of Biological Sciences,Beijing 102206,China;Beijing Advanced Innovation Center for Big Data-Based Precision Medicine,Beijing 100191,China;School of Biomedical Engineering,Capital Medical University,Beijing 100069,China;Chinese Institute for Brain Research,Beijing 102206,China;School of Life Sciences,Tsinghua University,Beijing 100084,China.

光电进展（英文版）

[1]Zhongyang Qi;Qingchun Guo;Shu Wang;Mingyue Jia;Xinwei Gao;Minmin Luo;Ling Fu-.All-fiber-transmission photometry for simultaneous optogenetic stimulation and multi-color neuronal activity recording)[J].光电进展（英文版）,2022(12):20-34,45

neuroscientists

All,fiber,transmission,photometry,simultaneous,optogenetic,stimulation,multi,color,neuronal,activity,recording,Manipulating,real,monitoring,activities,cell,type,specificity,precise,spatiotemporal,resolu,during,animal,behavior,are,fundamental,technologies,exploring,functional,connectivity,information,physiological,functions,neural,circuits,vivo,However,current,techniques,mostly,operate,independently,Here,report,all,system,manipulation,neurotransmitter,release,freely,moving,We,have,designed,manufactured,branch,bundle,enable,different,wavelengths,Further,combine,laser,narrow,linewidth,lock,amplification,method,suppress,induced,artifacts,channel,crosstalk,show,that,collection,efficiency,our,outperforms,traditional,epi,fluorescence,demonstrate,successful,DA,responses,unexpected,rewards,nucleus,accumbens,NAc,mouse,also,dual,Ca2+,signals,dynamics,upon,delivering,dopaminergic,terminals,same,location,Thus,provides,patible,efficient,flexible,solution,study,neurological,diseases

0.543168