Objective:The specific objective of this study was to evaluate the effects of atmospheric pressure plasma(APP)in the treatment of experimental periodontitis in Beagle dogs.Methods:The APP jet was diagnosed using optical emission spectroscopy and laser-induced fluorescence spectroscopy.Six Beagles received stainless steel ligatures to establish experimental periodontitis model.The teeth in the control group were subjected to conventional root surface debridement(RSD)and chlorhexidine irrigation.The APP group also started with RSD and was then subjected to plasma irradiation.Clinical analyses including plaque index,modified sulcus bleeding index,pocket depth and attachment loss(AL),as well as cone-beam computed tomography(CBCT)analysis,were performed at baseline,4th week,8th week and 12th week after treatment.Results:The results showed that typical reactive oxygen and nitrogen species were found in the full spectrum and the gas temperature of APP was close to room temperature.The highest concentrations of hydroxide and oxygen were obtained at 5 mm away from the nozzle.In both groups,all values in clinical examinations were significantly lower(P＜0.05)at 12th week after treatment than those at baseline.At the 12th week,the AL in clinical examinations and the bone loss in CBCT images in the APP group were significantly lower than those in the control group(P＜0.05).The hematoxylin-eosin staining showed more renascent alveolar bone in the APP group than in the control group.Conclusion:These findings suggested that APP has profound potential for use as an adjunct approach for periodontitis treatment.

Xue-zhi TANG;Jia-yin LI;Qi SHI;Han-yong ZHANG;Zhi-xiang ZHANG;Ke SONG;Xin-pei LU;Ying-guang CAO;Tian-feng DU

Department of Stomatology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China;State Key Laboratory of Advanced Electromagnetic Engineering and Technology,School of Electrical and Electronic Engineering,Huazhong University of Science and Technology,Wuhan 430074,China;Department of Stomatology,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China

当代医学科学(英文)

[1]Xue-zhi TANG;Jia-yin LI;Qi SHI;Han-yong ZHANG;Zhi-xiang ZHANG;Ke SONG;Xin-pei LU;Ying-guang CAO;Tian-feng DU-.Effects of Atmospheric Pressure Plasma in the Treatment of Experimental Periodontitis in Beagle Dogs)[J].当代医学科学(英文),2022(05):1079-1087

Beagles,ligatures,renascent

Effects,Atmospheric,Pressure,Plasma,Treatment,Experimental,Periodontitis,Dogs,Objective,specific,objective,this,study,was,evaluate,effects,atmospheric,pressure,plasma,treatment,experimental,periodontitis,dogs,Methods,jet,diagnosed,using,optical,emission,spectroscopy,laser,induced,fluorescence,Six,received,stainless,steel,establish,model,teeth,control,were,subjected,conventional,root,surface,debridement,RSD,chlorhexidine,irrigation,also,started,then,irradiation,Clinical,analyses,including,plaque,modified,sulcus,bleeding,pocket,depth,attachment,loss,AL,well,cone,beam,computed,tomography,CBCT,analysis,performed,baseline,4th,week,8th,12th,after,Results,results,showed,that,typical,reactive,oxygen,nitrogen,species,full,spectrum,gas,temperature,close,room,highest,concentrations,hydroxide,obtained,away,from,nozzle,In,both,groups,all,values,clinical,examinations,significantly,lower,than,those,bone,images,hematoxylin,eosin,staining,more,alveolar,Conclusion,These,findings,suggested,has,profound,potential,use,adjunct,approach

0.58482