典型文献
Screening of Seawater Compound Bioflocculant Producing Bacterial Strains and Optimization of Their Culture Medium
文献摘要：
Compared with single bioflocculant, compound bioflocculant ( CBF) normally has more stable and higher flocculation efficiencies. In order to produce a new compound bioflocculant, four bacterial strains with relative high rate of flocculation were selected from the coastal zone sediment, all of which were determined as Pseudomonas sp. by 16S rRNA sequencing. Through compounding, the ideal proportion of compound bioflocculant consisting of two bacterial strains ( F5 : M5) was determined as 1 : 2, resulting in 6％ increase in terms of flocculation rate. The significant fermentation factors in culture media and their limitations were investigated via single factor experiments and Plackett-Burman design, obtaining four key variables, MgSO4 , ( NH4 ) 2 SO4 , sucrose and peptone, which were further optimized by response surface methodology ( RSM) for their optimal concentrations. Finally, the ideal culture medium was determined, containing ( NH4) 2SO40.523 g, MgSO43.7 g, sucrose 12.82 g, K2HPO44 g, KH2PO43 g, NaCl 33 g, and deionized water 1 L. The flocculation rate of experimental data (88.92) agreed well with the model prediction, which was 16.60 higher than original rate.
文献关键词：
中图分类号：
作者姓名：
Kai Wang;Xujiao Ma;Siqi Li;Xiquan Cheng;Yingjie Zhang;Jun Ma
作者机构：
School of Marine Science and Technology,Harbin Institute of Technology(Weihai),Weihai 264209,China;School of Environment,Harbin Institute of Technology,Harbin 150090,China
文献出处：
引用格式：
[1]Kai Wang;Xujiao Ma;Siqi Li;Xiquan Cheng;Yingjie Zhang;Jun Ma-.Screening of Seawater Compound Bioflocculant Producing Bacterial Strains and Optimization of Their Culture Medium)[J].哈尔滨工业大学学报（英文版）,2022(03):1-12
A类：
Bioflocculant,bioflocculant,peptone,2SO40,MgSO43,K2HPO44
B类：
Screening,Seawater,Compound,Producing,Bacterial,Strains,Optimization,Their,Culture,Medium,Compared,single,CBF,normally,has,more,stable,higher,flocculation,efficiencies,In,order,produce,new,four,bacterial,strains,relative,rate,were,selected,from,coastal,zone,sediment,which,determined,Pseudomonas,by,16S,rRNA,sequencing,Through,compounding,ideal,proportion,consisting,two,F5,M5,was,resulting,increase,terms,significant,fermentation,factors,culture,media,their,limitations,investigated,via,experiments,Plackett,Burman,design,obtaining,key,variables,NH4,sucrose,further,optimized,response,surface,methodology,RSM,optimal,concentrations,Finally,medium,containing,KH2PO43,NaCl,deionized,experimental,data,agreed,well,model,prediction,than,original
AB值：
0.587502
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