典型文献
Visual Detection of Vibrio parahaemolyticus using Combined CRISPR/Cas12a and Recombinase Polymerase Amplification
文献摘要：
Objective To establish an ultra-sensitive, ultra-fast, visible detection method for Vibrio parahaemolyticus (VP). Methods We established a new method for detecting the tdh and trh genes of VP using clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 12a (CRISPR/Cas12a) combined with recombinase polymerase amplification and visual detection (CRISPR/Cas12a-VD). Results CRISPR/Cas12a-VD accurately detected target DNA at concentrations as low as 10-18 M (single molecule detection) within 30 min without cross-reactivity against other bacteria. When detecting pure cultures of VP, the consistency of results reached 100％ compared with real-time PCR. The method accurately analysed pure cultures and spiked shrimp samples at concentrations as low as 102 CFU/g. Conclusion The novel CRISPR/Cas12a-VD method for detecting VP performed better than traditional detection methods, such as real-time PCR, and has great potential for preventing the spread of pathogens.
文献关键词：
中图分类号：
作者姓名：
JIANG Han Ji;TAN Rong;JIN Min;YIN Jing;GAO Zhi Xian;LI Hai Bei;SHI Dan Yang;ZHOU Shu Qing;CHEN Tian Jiao;YANG Dong;LI Jun Wen
作者机构：
Tianjin Institute of Environmental&Operational Medicine,Tianjin 300050,China
文献出处：
引用格式：
[1]JIANG Han Ji;TAN Rong;JIN Min;YIN Jing;GAO Zhi Xian;LI Hai Bei;SHI Dan Yang;ZHOU Shu Qing;CHEN Tian Jiao;YANG Dong;LI Jun Wen-.Visual Detection of Vibrio parahaemolyticus using Combined CRISPR/Cas12a and Recombinase Polymerase Amplification)[J].生物医学与环境科学（英文版）,2022(06):518-527
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B类：
Visual,Detection,Vibrio,parahaemolyticus,using,Combined,CRISPR,Cas12a,Recombinase,Polymerase,Amplification,Objective,To,ultra,sensitive,fast,visible,detection,VP,Methods,We,established,new,detecting,tdh,trh,genes,clustered,regularly,interspaced,short,palindromic,repeats,associated,protein,combined,recombinase,polymerase,amplification,visual,VD,Results,accurately,detected,target,concentrations,low,single,molecule,within,without,cross,reactivity,against,other,bacteria,When,pure,cultures,consistency,results,reached,compared,real,analysed,spiked,shrimp,samples,CFU,Conclusion,novel,performed,better,than,traditional,methods,such,has,great,potential,preventing,spread,pathogens
AB值：
0.615101
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