典型文献
Arctigenin attenuates paraquat-induced human lung epithelial A549 cell injury by suppressing ROS/p38 mitogen-activated protein kinases-mediated apoptosis
文献摘要：
BACKGROUND: Paraquat (PQ)-induced acute lung injury (ALI) and pulmonary fi brosis are common diseases with high mortality but without eff ective antidotes in emergency medicine. Our previous study has proved that arctigenin suppressed pulmonary fibrosis induced by PQ. We wondered whether arctigenin could also have a protective eff ect on PQ-induced ALI.METHODS: A PQ-induced A549 cell injury model was used, and the effect of arctigenin was determined by a cell counting kit-8 (CCK-8) cell viability assay. In addition, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labelling (TUNEL) staining assays and mitochondrial membrane potential assays were performed to evaluate the level of cell apoptosis. The generation of reactive oxygen species (ROS) was refl ected by dihydroethidium (DHE) staining and a 2',7'-dichlorodihy drofluorescein diacetate (DCFH-DA) assay. Moreover, immunoblotting studies were used to assess the expression of mitogen-activated protein kinases (MAPKs) and p38 MAPK. RESULTS: Arctigenin attenuated PQ-induced inhibition of A549 cell viability in a dose-dependent manner. Arctigenin also significantly reduced PQ-induced A549 cell apoptosis, as refl ected by the TUNEL assay and mitochondrial membrane potential assay, which may result from suppressed ROS/p38 MAPK signaling because we found that arctigenin dramatically suppressed ROS generation and p38 MAPK phosphorylation. CONCLUSION: Arctigenin could attenuate PQ-induced lung epithelial A549 cell injury in vitro by suppressing ROS/p38 MAPK-mediated cell apoptosis, and arctigenin might be considered a potential candidate drug for PQ-induced ALI.
文献关键词：
中图分类号：
作者姓名：
Chao Liu;Zhao-rui Sun;Meng-meng Wang;Zhi-zhou Yang;Wei Zhang;Yi Ren;Xiao-qin Han;Rui Liu;Quan Li;Shi-nan Nie
作者机构：
Department of Emergency Medicine,Jinling Hospital,Medical School of Nanjing University,Nanjing 210002,China
文献出处：
引用格式：
[1]Chao Liu;Zhao-rui Sun;Meng-meng Wang;Zhi-zhou Yang;Wei Zhang;Yi Ren;Xiao-qin Han;Rui Liu;Quan Li;Shi-nan Nie-.Arctigenin attenuates paraquat-induced human lung epithelial A549 cell injury by suppressing ROS/p38 mitogen-activated protein kinases-mediated apoptosis)[J].世界急诊医学杂志（英文）,2022(05):373-378
A类：
Arctigenin,brosis,antidotes,arctigenin,refl,dichlorodihy,drofluorescein
B类：
attenuates,paraquat,induced,human,lung,epithelial,A549,cell,injury,by,suppressing,ROS,p38,mitogen,activated,protein,kinases,mediated,apoptosis,BACKGROUND,Paraquat,PQ,acute,ALI,pulmonary,are,common,diseases,high,mortality,but,without,emergency,medicine,Our,previous,study,has,proved,that,suppressed,fibrosis,We,wondered,whether,could,also,have,protective,METHODS,model,was,used,effect,determined,counting,kit,CCK,viability,In,addition,terminal,deoxynucleotidyl,transferase,TdT,dUTP,nick,labelling,TUNEL,staining,assays,mitochondrial,membrane,potential,were,performed,evaluate,level,generation,reactive,oxygen,species,ected,dihydroethidium,DHE,diacetate,DCFH,DA,Moreover,immunoblotting,studies,assess,expression,MAPKs,RESULTS,attenuated,inhibition,dose,dependent,manner,significantly,reduced,which,may,result,from,signaling,because,found,dramatically,phosphorylation,CONCLUSION,vitro,might,considered,candidate,drug
AB值：
0.480513
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