典型文献
Establishment and Characterization of a Fin Cell Line Derived from the Atlantic Salmon Salmo salar and Its Application to Fish Virology Study
文献摘要：
Atlantic salmon(Salmo salar)is an important economic fish that is seriously threatened by various viruses.A cell line designated as ASF derived from the caudal fin tissue of Atlantic salmon was established and characterized in this study.ASF cells grew well in Dulbecco's modified Eagle's medium(DMEM)containing 20％fetal bovine serum at 20℃.DNA sequencing and com-parative analysis of the cytochrome B gene verified that the ASF cell line originated from Atlantic salmon.Chromosome analysis indicated that the modal chromosome number of ASF cells was 58.Viral susceptibility test showed that ASF cells were susceptive to two important fish viruses,viral hemorrhagic septicemia virus(VHSV)and red-spotted grouper nervous necrosis virus(RGNNV).Viral replication in ASF cells was further confirmed by qRT-PCR and transmission electron microscopy.Moreover,VHSV and RGNNV infections could induce the cellular responses in ASF cells,as indicated by the differential expression of cellular antiviral response-related genes,interferon-1 and Mx-1.In conclusion,the newly established ASF cell line can be applied as an in vitro tool in fish virology and immunity studies.
文献关键词：
中图分类号：
作者姓名：
JIA Peng;LIN Lirong;XU La;YI Meisheng;JIA Kuntong
作者机构：
School of Marine Sciences,Sun Yat-sen University,Guangzhou 510000,China;Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai),Zhuhai 519082,China;Zhuhai Key Laboratory of Marine Bioresources and Environment,Sun Yat-sen University,Guangzhou 510000,China;Marine Biology Institute of Shandong Province;Shandong Key Laboratory of Disease Control in Mariculture,Qingdao 266104, China
文献出处：
引用格式：
[1]JIA Peng;LIN Lirong;XU La;YI Meisheng;JIA Kuntong-.Establishment and Characterization of a Fin Cell Line Derived from the Atlantic Salmon Salmo salar and Its Application to Fish Virology Study)[J].中国海洋大学学报（自然科学英文版）,2022(04):946-954
A类：
susceptive,VHSV
B类：
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AB值：
0.602688
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