A recombinant inbred line(RIL)with 179 lines derived from a cross between a heat tolerant line Huhan 1B and a heat sensitive line Hanhui 3 was used for QTL mapping for panicle traits and heat tolerance.A total of 52 QTLs were identified with LOD scores from 3.85 to 54.93,each explaining 0.70％to 21.48％of the phenotypic variances,including 11 for panicle length(PL),11 for seed number per panicle(SN),14 for spikelet number per panicle(SNPP),6 for seed-setting rate(SSR)under normal conditions,and 5 for seed-setting rate(SSR HT)and 5 for relative seed-setting rate(RSSR)under heat stress,respectively.OsHSP70.1 located in qRSSR1 interval was predicted as a heat tolerant candidate gene.Haplotype analysis revealed the indica-japonica differentiation of OsHSP70.1,implying possible relation to temperature adaptability in rice.An insertion/deletion(InDel)marker HSP70-12 for OsHSP70.1 and a cleavage-amplified polymorphic sequence(CAPS)marker SPKIE-299 for NAL1/SPIKE were developed,and validated as co-segregating markers with heat tolerance or panicle traits in different populations.These two markers can be used to facilitate rice grain yield improvement or heat tolerance via marker-assisted selection(MAS)breeding.

LIU Hongyan;MA Xiaosong;LI Enxi;ZENG Xianjun;LUO Lijun

Shanghai Agrobiological Gene Center,Shanghai 201106,China;Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization,Ministry of Agriculture and Rural Affairs,Shanghai 201106,China;Hainan University,Haikou 570228,China;Huazhong Agricultural University,Wuhan 430070,China

水稻科学（英文版）

[1]LIU Hongyan;MA Xiaosong;LI Enxi;ZENG Xianjun;LUO Lijun-.Genetic Dissection of Quantitative Trait Loci for Panicle Traits and Heat Tolerance by High-Density Bin Map in Rice)[J].水稻科学（英文版）,2022(06):507-511,中插6-中插19

Loci,Huhan,Hanhui,SNPP,OsHSP70,qRSSR1,SPKIE,SPIKE

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