典型文献
A Simple and Rapid Oxidative Stress Screening Method of Small Molecules for Functional Studies of Transcription Factor
文献摘要：
Structure-assisted reverse chemical discovery approaches are becoming attractive due to target based screening.However,Small molecules(SMs)identified by reverse chemical genetic approach require robust phenotyping assays to study the plant responses.We optimized a screening method in rice seedlings using methyl viologen(MV)induced oxidative stress under high light conditions.This method was suitable for studying the efficacy of SM targeting oxidative stress related genes.Structure-assisted drug designing approach was used to identify SM targeting DREB2A transcription factor(TF).Rice seedlings treated with pronetalol showed susceptible phenotype when exposed to oxidative stress.Pronetalol inhibited the DREB2A activity and suppressed the expression of its target LEA 7 and HSFA3 genes under oxidative stress.The assay is quite simple and robust and can be expanded to high throughput screening method for functional validation of TF.
文献关键词：
中图分类号：
作者姓名：
Vanitha ADHI NARAYAN REDDY;Preethi VIJAYARAGHAVAREDDY;Ashwin VARGHEESE;Sujitha DADI;Akshay UTTARKAR;Vidya NIRANJAN;Anuradha VENKATRAMAN;Sheshshayee M.SREEMAN;Ramu S.VEMANNA
作者机构：
Department of Crop Physiology,University of Agricultural Sciences,Bengaluru 560065,India;Department of Biochemistry&Biotechnology,Annamalai University,Chidambaram 608002,India;Department of Biotechnology,Rashtreeya Vidyalaya,College of Engineering,Bengaluru 560059,India;Laboratory of Plant Functional Genomics,Regional Centre for Biotechnology,Faridabad-Gurgaon Expressway,Faridabad 121001,India
文献出处：
引用格式：
[1]Vanitha ADHI NARAYAN REDDY;Preethi VIJAYARAGHAVAREDDY;Ashwin VARGHEESE;Sujitha DADI;Akshay UTTARKAR;Vidya NIRANJAN;Anuradha VENKATRAMAN;Sheshshayee M.SREEMAN;Ramu S.VEMANNA-.A Simple and Rapid Oxidative Stress Screening Method of Small Molecules for Functional Studies of Transcription Factor)[J].水稻科学（英文版）,2022(05):402-406,中插12-中插16
A类：
DREB2A,pronetalol,Pronetalol,HSFA3
B类：
Simple,Rapid,Oxidative,Stress,Screening,Method,Small,Molecules,Functional,Studies,Transcription,Factor,Structure,assisted,reverse,chemical,discovery,approaches,are,becoming,attractive,due,screening,However,molecules,SMs,identified,by,genetic,require,robust,phenotyping,assays,plant,responses,We,optimized,method,rice,seedlings,using,methyl,viologen,MV,induced,oxidative,stress,under,high,light,conditions,This,was,suitable,studying,efficacy,targeting,related,genes,drug,designing,used,identify,transcription,TF,Rice,treated,showed,susceptible,phenotype,when,exposed,inhibited,activity,suppressed,expression,its,LEA,quite,simple,can,expanded,throughput,functional,validation
AB值：
0.623612
相似文献
机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
