典型文献
Reprogramming Mycobacterium tuberculosis CRISPR System for Gene Editing and Genome-wide RNA Interference Screening
文献摘要：
Mycobacterium tuberculosis is the causative agent of tuberculosis(TB),which is still the leading cause of mortality from a single infectious disease worldwide.The development of novel anti-TB drugs and vaccines is severely hampered by the complicated and time-consuming genetic manipulation techniques for M.tuberculosis.Here,we harnessed an endogenous type Ⅲ-A CRISPR/Cas10 system of M.tuberculosis for efficient gene editing and RNA interference(RNAi).This simple and easy method only needs to transform a single mini-CRISPR array plasmid,thus avoiding the introduction of exogenous protein and minimizing proteotoxicity.We demonstrated that M.tuberculosis genes can be efficiently and specifically knocked in/out by this system as con-firmed by DNA high-throughput sequencing.This system was further applied to single-and multiple-gene RNAi.Moreover,we successfully performed genome-wide RNAi screening to identify M.tuberculosis genes regulating in vitro and intracellular growth.This system can be extensively used for exploring the functional genomics of M.tuberculosis and facilitate the development of novel anti-TB drugs and vaccines.
文献关键词：
中图分类号：
作者姓名：
Khaista Rahman;Muhammad Jamal;Xi Chen;Wei Zhou;Bin Yang;Yanyan Zou;Weize Xu;Yingying Lei;Chengchao Wu;Xiaojian Cao;Rohit Tyagi;Muhammad Ahsan Naeem;Da Lin;Zeshan Habib;Nan Peng;Zhen F.Fu;Gang Cao
作者机构：
State Key Laboratory of Agricultural Microbiology,Huazhong Agricultural University,Wuhan 430070,China;College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,China;Bio-Medical Center,Huazhong Agricultural University,Wuhan 430070,China;Key Laboratory of Preventive Veterinary Medicine in Hubei Province,the Cooperative Innovation Center for Sustainable Pig Production,Wuhan 430070,China
文献出处：
引用格式：
[1]Khaista Rahman;Muhammad Jamal;Xi Chen;Wei Zhou;Bin Yang;Yanyan Zou;Weize Xu;Yingying Lei;Chengchao Wu;Xiaojian Cao;Rohit Tyagi;Muhammad Ahsan Naeem;Da Lin;Zeshan Habib;Nan Peng;Zhen F.Fu;Gang Cao-.Reprogramming Mycobacterium tuberculosis CRISPR System for Gene Editing and Genome-wide RNA Interference Screening)[J].基因组蛋白质组与生物信息学报（英文版）,2022(06):1180-1196
A类：
Reprogramming,Cas10,proteotoxicity
B类：
Mycobacterium,tuberculosis,CRISPR,System,Gene,Editing,Genome,Interference,Screening,causative,agent,TB,which,still,leading,cause,mortality,from,single,infectious,disease,worldwide,development,novel,anti,drugs,vaccines,severely,hampered,by,complicated,consuming,genetic,manipulation,techniques,Here,we,harnessed,endogenous,type,system,editing,interference,RNAi,This,simple,easy,method,only,needs,transform,array,plasmid,thus,avoiding,introduction,exogenous,protein,minimizing,We,demonstrated,that,genes,can,efficiently,specifically,knocked,out,this,firmed,high,throughput,sequencing,was,further,applied,multiple,Moreover,successfully,performed,genome,screening,identify,regulating,vitro,intracellular,growth,extensively,used,exploring,functional,genomics,facilitate
AB值：
0.596214
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