典型文献
Enhanced oxidase-like activity of g-C3N4 nanosheets supported Pd nanosheets for ratiometric fluorescence detection of acetylcholinesterase activity and its inhibitor
文献摘要：
The undesirable enzymatic activity of nanozymes under near neutral pH condition and the traditional single signal output always restrict the analytical application of nanozyme-based biosensors.Herein,graphitic carbon nitride nanosheets supported palladium nanosheets composite(Pd/g-C3N4)with both oxidase-like activity and fluorescent property is synthesized.Notably,Pd/g-C3N4 exhibits enhanced oxidase-like activity compared to Pd NSs under pH 7.4.By combining Pd/g-C3N4 with o-phenylenediamine(OPD),a ratiometric fluorescence assay for acetylcholinesterase(AChE)activity detection is developed.Pd/g-C3N4 can catalyze oxidation of nonfluorescent OPD to fluorescent oxidized OPD(oxOPD,Em=565 nm),which can quench fluorescence of g-C3N4 supporter(Em=441 nm)through fluorescence reso-nance energy transfer(FRET).However,in presence of AChE,acetylthiocholine can be hydrolyzed into thiocholine,which will block the oxidase-like activity of Pd/g-C3N4 and then hamper the FRET process.This ratiometric fluorescence assay is also viable to screen AChE inhibitor.This work will guide design of ratiometric fluorescence assay based on nanozymes with improved enzymatic activity.
文献关键词：
中图分类号：
作者姓名：
Chenghui Zhang;Pengjuan Ni;Bo Wang;Wendong Liu;Yuanyuan Jiang;Chuanxia Chen;Jian Sun;Yizhong Lu
作者机构：
School of Materials Science and Engineering,University of Jinan,Ji'nan 250022,China;State Key Laboratory of Electroanalytical Chemistry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China
文献出处：
引用格式：
[1]Chenghui Zhang;Pengjuan Ni;Bo Wang;Wendong Liu;Yuanyuan Jiang;Chuanxia Chen;Jian Sun;Yizhong Lu-.Enhanced oxidase-like activity of g-C3N4 nanosheets supported Pd nanosheets for ratiometric fluorescence detection of acetylcholinesterase activity and its inhibitor)[J].中国化学快报（英文版）,2022(02):757-761
A类：
nonfluorescent,oxOPD,acetylthiocholine,thiocholine
B类：
Enhanced,oxidase,like,activity,C3N4,nanosheets,supported,Pd,ratiometric,fluorescence,detection,acetylcholinesterase,inhibitor,undesirable,enzymatic,nanozymes,under,near,neutral,condition,traditional,single,signal,output,always,restrict,analytical,application,biosensors,Herein,graphitic,carbon,nitride,palladium,composite,both,property,synthesized,Notably,exhibits,enhanced,compared,NSs,By,combining,phenylenediamine,assay,AChE,developed,can,catalyze,oxidation,oxidized,Em,which,quench,supporter,through,reso,nance,energy,transfer,FRET,However,presence,be,hydrolyzed,into,will,block,then,hamper,process,This,also,viable,screen,work,guide,design,improved
AB值：
0.493734
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