典型文献
Construction of chimeric viruses based on pepper mild mottle virus using a modified Cre/loxP system
文献摘要：
Cre/loxP, a site-specific recombination system, has been widely used for various purposes, including chromosomal translocations, generation of marker-free transgenic plants, tissue-specific activation of a reporter gene and efficient heterologous gene expression in plants. However, stable or transient expression of Cre recombinase in plants can cause chlorosis or necrosis. Here, we describe a modified Cre/loxP recombination system using a DNA fragment flanked with loxP sites in the same orientation in which necrosis induced by Cre recombinase in Nicotiana benthamiana leaves was alleviated. The modified system was successfully used to create functional GFP-tagged pepper mild mottle virus (PMMoV) and a chimeric virus with coat protein (CP) substitution assembled from separate pro-vector modules. Our results provide a new strategy and flexible technique to construct chimeric virus and infectious clones for plant viruses with large genomes.
文献关键词：
中图分类号：
作者姓名：
YIN Yue-yan;HUA Meng-ying;ZHAO Kuang-jie;WAN Qiong-lian;BU Shan;LU Yu-wen;ZHENG Hong-ying;RAO Shao-fei;YAN Fei;PENG Jie-jun;CHEN Hai-ru;CHEN Jian-ping
作者机构：
College of Plant Protection,Yunnan Agricultural University,Kunming 650201,P.R.China;Institute of Alpine Economic Plants,Yunnan Academy of Agricultural Sciences,Lijiang 674100,P.R.China;State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agroproducts,Institute of Plant Virology,Ningbo University,Ningbo 315211,P.R.China;Longping Branch of Graduate College,Hunan University,Changsha 410125,P.R.China
文献出处：
引用格式：
[1]YIN Yue-yan;HUA Meng-ying;ZHAO Kuang-jie;WAN Qiong-lian;BU Shan;LU Yu-wen;ZHENG Hong-ying;RAO Shao-fei;YAN Fei;PENG Jie-jun;CHEN Hai-ru;CHEN Jian-ping-.Construction of chimeric viruses based on pepper mild mottle virus using a modified Cre/loxP system)[J].农业科学学报（英文）,2022(08):2456-2463
A类：
PMMoV
B类：
Construction,chimeric,viruses,pepper,mild,mottle,using,modified,Cre,loxP,system,specific,recombination,has,been,widely,used,various,purposes,including,chromosomal,translocations,generation,marker,free,transgenic,plants,tissue,activation,reporter,efficient,heterologous,expression,However,stable,transient,recombinase,can,cause,chlorosis,necrosis,Here,describe,fragment,flanked,sites,same,orientation,which,induced,by,Nicotiana,benthamiana,leaves,was,alleviated,successfully,create,functional,GFP,tagged,coat,protein,CP,substitution,assembled,from,separate,vector,modules,Our,results,provide,new,strategy,flexible,technique,construct,infectious,clones,large,genomes
AB值：
0.617466
相似文献
机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
