Hepatitis B virus(HBV)genotype B and C are two major genotypes that are prevalent in Asia and differ in natural history and disease progression.The impact of HBV genotypes on viral replication and protein expression has been explored by the transfection of hepatoma cells with replication-competent HBV DNA,which mimics the later stages of the viral life cycle.However,the influence of HBV genotypes on the early events of viral infection re-mains undetermined,mainly due to the difficulties in obtaining sufficient infectious viral particles for infection assays.Here,we report that a high-titer HBV inoculum can be generated from the transient transfection-based cell model after optimizing transfection conditions and modifying the HBV-expressing construct.By performing in vitro infection assays using transiently transfected derived viruses,we found that clinical genotype C isolates possessed higher infectivity than genotype B isolates.Moreover,we identified a naturally occurring mutation sL21S in small hepatitis B surface protein,which markedly decreased the infectivity of HBV genotype C isolates,but not that of genotype B isolates.In summary,using infectious viral particles provided by the optimized transient transfection-based cell model,we have been able to investigate a wide range of HBV variants on viral infectivity,which may contribute to our understanding of the reasons for different clinical outcomes in HBV infections and the development of therapeutic drugs targeting the early stages of HBV life cycle.

Tingting Liu;Anlei Liu;Yong Liu;Shan Cen;Quan Zhang

Department of Transfusion Medicine,Nanjing Drum Tower Hospital,The Affiliated Hospital of Nanjing University Medical School,Nanjing,210008,China;Department of Emergency Medicine,Peking Union Medical College Hospital,Chinese Academy of Medical Science,Beijing,100730,China;Department of Laboratory Medicine,Nanjing Drum Tower Hospital and Jiangsu Key Laboratory for Molecular Medicine,The Affiliated Hospital of Nanjing University Medical School,Nanjing,210008,China;Institute of Medicinal Biotechnology,Chinese Academy of Medical Science,Beijing,100050,China;Department of Infectious Diseases,Nanjing Drum Tower Hospital,The Affiliated Hospital of Nanjing University Medical School,Nanjing,210008,China

中国病毒学

[1]Tingting Liu;Anlei Liu;Yong Liu;Shan Cen;Quan Zhang-.In vitro investigation of HBV clinical isolates from Chinese patients reveals that genotype C isolates possess higher infectivity than genotype B isolates)[J].中国病毒学,2022(03):398-407

sL21S

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