典型文献
Generation and application of two monoclonal antibodies targeting conserved linear epitopes in the NP protein of influenza A virus
文献摘要：
Monoclonal antibodies (mAbs) are widely used in virus research and disease diagnosis. The nucleoprotein (NP) of influenza A virus (IAV) plays important roles in multiple stages of the virus life cycle. Therefore, generating conserved mAbs against NP and characterizing their properties will provide useful tools for IAV research. In this study, two mAbs against the NP protein, 10E9 and 3F3, were generated with recombinant truncated NP proteins (NP-1 and NP-2) as immunogens. The heavy-chain subclass of both 10E9 and 3F3 was determined to be IgG2α, and the light-chain type was κ. Truncation and site-specific mutation analyses showed that the epitopes of mAbs 10E9 and 3F3 were located in the N terminal 84–89 amino acids and the C terminal 320–324 amino acids of the NP protein, respectively. We found that mAbs 10E9 and 3F3 reacted well with the NP protein of H1–H15 subtypes of IAV. Both 10E9 and 3F3 can be used in immunoprecipitation assay, and 10E9 was also successfully applied in confocal microscopy. Furthermore, we found that the 10E9-recognized 84SAGKDP89 epitope and 3F3-recognized 320ENPAH324 epitope were highly conserved in NP among all avian and human IAVs. Thus, the two mAbs we developed could be used as powerful tools in the development of diagnostic methods of IAV, and also surely promote the basic research in understanding the replication mechanisms of IAV.
文献关键词：
中图分类号：
作者姓名：
ZHAO Yu-hui;WEN Xia;LI Qi-bing;JIANG Li;WANG Guang-wen;LIANG Li-bin;WANG Xiu-rong;CHEN Hua-lan;LI Cheng-jun
作者机构：
State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150069,P.R.China
文献出处：
引用格式：
[1]ZHAO Yu-hui;WEN Xia;LI Qi-bing;JIANG Li;WANG Guang-wen;LIANG Li-bin;WANG Xiu-rong;CHEN Hua-lan;LI Cheng-jun-.Generation and application of two monoclonal antibodies targeting conserved linear epitopes in the NP protein of influenza A virus)[J].农业科学学报（英文）,2022(07):2095-2105
A类：
10E9,immunogens,Truncation,84SAGKDP89,320ENPAH324,IAVs
B类：
Generation,application,two,monoclonal,antibodies,targeting,conserved,linear,epitopes,influenza,virus,Monoclonal,mAbs,are,widely,used,research,disease,diagnosis,nucleoprotein,plays,important,roles,multiple,stages,life,cycle,Therefore,generating,against,characterizing,their,properties,will,provide,useful,tools,In,this,study,3F3,were,generated,recombinant,truncated,proteins,heavy,chain,subclass,both,was,determined,be,IgG2,light,site,specific,mutation,analyses,showed,that,located,terminal,amino,acids,respectively,We,found,reacted,well,H15,subtypes,Both,can,immunoprecipitation,assay,also,successfully,applied,confocal,microscopy,Furthermore,recognized,highly,among,all,avian,human,Thus,developed,could,powerful,development,diagnostic,methods,surely,promote,basic,understanding,replication,mechanisms
AB值：
0.456626
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