典型文献
A novel non-invasive identification of genome editing mutants from insect exuviae
文献摘要：
With the wide application of genome editing in insects,a simple and efficient identification method is urgently needed to meet the increasing demand for mutation de-tection.Here,taking migratory locusts as a model system,we developed a non-invasive method to accurately identify genome-edited mutants by using DNA from insect exuviae.We compared the quantity and quality of genomic DNA from exuviae in five instar hop-pers and found that the 1 st instar exuviae had the highest DNA yield and content,while the 3rd instar exuviae had the best quality.Consensus genotypes were identified from genomic DNA of hoppers at different developmental stages in the same individuals.Moreover,we demonstrated that the amplification products from DNA extracted from locust exuviae are the consensus sequences with those from the hemolymph and foreleg pre-tarsus.There-fore,non-invasive samples provide the same genotyping results as minimally invasive and invasive samples of the same individuals.Furthermore,this identification method that uses genomic DNA from exuviae can be used for early screening of positive genome-edited in-dividuals in each generation for adult crossing.In our study,the non-invasive identification method was not only simpler and provided results earlier than existing methods,but also had a better reproducibility and accuracy.This non-invasive identification approach us-ing genomic DNA from exuviae can be adapted to meet the growing demand for genetic analysis and will find wide application in insect genome editing research.
文献关键词：
中图分类号：
作者姓名：
Xiao-Xiao Wang;Jing Li;Tong-Xin Wang;Yi-Nuo Yang;Hai-Kang Zhang;Meng Zhou;Le Kang;Li-Ya Wei
作者机构：
College of Life Sciences,Institute of Life Sciences and Green Development,Hebei University,Baoding,Hebei Province,071002,China;State Key Laboratory of Integrated Management of Pest Insects and Rodents,Institute of Zoology,Chinese Academy of Sciences,Beijing,100101,China
文献出处：
引用格式：
[1]Xiao-Xiao Wang;Jing Li;Tong-Xin Wang;Yi-Nuo Yang;Hai-Kang Zhang;Meng Zhou;Le Kang;Li-Ya Wei-.A novel non-invasive identification of genome editing mutants from insect exuviae)[J].中国昆虫科学（英文版）,2022(01):21-32
A类：
exuviae,hoppers,foreleg
B类：
novel,invasive,identification,genome,editing,mutants,from,With,wide,application,insects,efficient,urgently,needed,meet,increasing,demand,mutation,tection,Here,taking,migratory,locusts,model,system,developed,accurately,identify,edited,by,using,We,compared,quantity,quality,genomic,five,instar,found,that,had,highest,yield,content,while,3rd,best,Consensus,genotypes,were,identified,different,developmental,stages,same,individuals,Moreover,demonstrated,amplification,products,extracted,consensus,sequences,those,hemolymph,pre,tarsus,There,samples,genotyping,results,minimally,Furthermore,this,uses,can,used,early,screening,positive,each,generation,adult,crossing,In,our,study,was,only,simpler,provided,earlier,than,existing,methods,but,also,better,reproducibility,accuracy,This,approach,adapted,growing,genetic,analysis,will,find,research
AB值：
0.472945
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