典型文献
Changes in chemical composition,structural and functional microbiome during alfalfa(Medicago sativa)ensilage with Lactobacillus plantarum PS-8
文献摘要:
Improving silage production by adding exogenous microorganisms not only maximizes nutrient pres-ervation,but also extends product shelf life.Herein,changes in the quality and quantity of Lactobacillus plantarum PS-8(PS-8)-inoculated alfalfa(Medicago sativa)during silage fermentation were monitored at d 0,7,14,and 28(inoculum dose of PS-8 was 1 x 105 colony forming units[cfu]/g fresh weight;50 kg per bag;10 bags for each time point)by reconstructing metagenomic-assembled genomes(MAG)and Growth Rate InDex(GRiD).Our results showed that the exogenous starter bacterium,PS-8 inoculation,became the most dominating strain by d 7,and possibly played a highly active role throughout the fermentation process.The pH value of the silage decreased greatly,accompanied by the growth of acid-producing microorganisms namely PS-8,which inhibited the growth of harmful microorganisms like molds(4.18 vs.1.42 log cfu/g)and coliforms(4.95 vs.0.66 log most probable number[MPN]/g).The content of neutral detergent fiber(NDF)decreased significantly(41.6%vs.37.6%;dry matter basis).In addition,the abundance and diversity of genes coding microbial carbohydrate-active enzymes(CAZymes)increased significantly and desirably throughout the fermentation,particularly the genes responsible for degrading starch,arabino-xylan,and cellulose.Overall,our results showed that PS-8 was replicating rapidly and consistently during early-and mid-fermentation phases,promoting the growth of beneficial lactic acid bacteria and inhibiting undesirable microbes,ultimately improving the quality of silage.
文献关键词:
中图分类号:
作者姓名:
Lijun You;Weichen Bao;Caiqing Yao;Feiyan Zhao;Hao Jin;Weiqiang Huang;Bohai Li;Lai-Yu Kwok;Wenjun Liu
作者机构:
Key Laboratory of Dairy Biotechnology and Engineering,Ministry of Education,Key Laboratory of Dairy Products Processing,Ministry of Agriculture and Rural Affairs,Inner Mongolia Agricultural University,Hohhot,010018,China
文献出处:
引用格式:
[1]Lijun You;Weichen Bao;Caiqing Yao;Feiyan Zhao;Hao Jin;Weiqiang Huang;Bohai Li;Lai-Yu Kwok;Wenjun Liu-.Changes in chemical composition,structural and functional microbiome during alfalfa(Medicago sativa)ensilage with Lactobacillus plantarum PS-8)[J].动物营养(英文),2022(02):100-109
A类:
ensilage,ervation,InDex,GRiD,coliforms,desirably,arabino
B类:
Changes,chemical,composition,structural,functional,microbiome,during,alfalfa,Medicago,sativa,Lactobacillus,plantarum,PS,Improving,production,by,adding,exogenous,microorganisms,not,only,maximizes,nutrient,pres,but,also,extends,shelf,life,Herein,changes,quality,quantity,inoculated,fermentation,were,monitored,inoculum,dose,was,colony,forming,units,cfu,fresh,weight,per,bags,each,point,reconstructing,metagenomic,assembled,genomes,MAG,Growth,Rate,Our,results,showed,that,starter,bacterium,inoculation,became,most,dominating,strain,possibly,played,highly,active,role,throughout,process,value,decreased,greatly,accompanied,growth,acid,producing,namely,which,inhibited,harmful,like,molds,log,probable,number,MPN,content,neutral,detergent,fiber,NDF,significantly,dry,matter,basis,addition,abundance,diversity,genes,coding,microbial,carbohydrate,enzymes,CAZymes,increased,particularly,responsible,degrading,starch,xylan,cellulose,Overall,our,replicating,rapidly,consistently,early,mid,phases,promoting,beneficial,lactic,bacteria,inhibiting,undesirable,microbes,ultimately,improving
AB值:
0.642152
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