Nutritional stimulation of the developing small intestine of chick embryos can be conducted by in-ovo feeding(IOF).We hypothesized that IOF of glutamine and leucine can enhance small intestinal devel-opment by promoting proliferation and differentiation of multipotent small intestinal epithelial cells.Broiler embryos(n=128)were subject to IOF of glutamine(IOF-Gln),leucine(IOF-Leu),NaCl(IOF-NaCl)or no injection(control)at embryonic d 17(E 17).Multipotent,progenitor and differentiated cells were located and quantified in the small intestinal epithelium between E 17 and d 7 after hatch(D 7)in all treatment groups by immunofluorescence of SRY-box transcription factor 9(Sox9)and proliferating cell nuclear antigen(PCNA),in-situ hybridization of leucine-rich repeat containing G-protein coupled receptor 5(Lgr5)and peptide transporter 1(PepT1)and histochemical goblet cell staining.The effects of IOF treatments at E 19(48 h post-IOF),in comparison to control embryos,were as follows:total cell counts increased by 40％,33％and 19％,and multipotent cell counts increased by 52％,50％and 38％,in IOF-Gln,IOF-Leu and IOF-NaCl embryos,respectively.Only IOF-Gln embryos exhibited a significance,36％in-crease in progenitor cell counts.All IOF treatments shifted Lgr5+stem cell localizations to villus bottoms.The differentiated,PepT1+region of the villi was 1.9 and 1.3-fold longer in IOF-Gln and IOF-Leu embryos,respectively,while goblet cell densities decreased by 20％in IOF-Gln embryos.Post-hatch,crypt and villi epithelial cell counts were significantly higher IOF-Gln chicks,compared to control chicks(P＜0.05).We conclude IOF of glutamine stimulates small intestinal maturation and functionality during the peri-hatch period by promoting multipotent cell proliferation and differentiation,resulting in enhanced compart-mentalization of multipotent and differentiated cell niches and expansions of the absorptive surface area.

Naama Reicher;Tal Melkman-Zehavi;Jonathan Dayan;Eric A.Wong;Zehava Uni

Department of Animal Science,The Robert H.Smith,Faculty of Agriculture,Food and Environment,The Hebrew University of Jerusalem,Rehovot 76100,Israel;Department of Animal and Poultry Sciences,Virginia Tech,Blacksburg,VA 24061,USA

动物营养（英文）

[1]Naama Reicher;Tal Melkman-Zehavi;Jonathan Dayan;Eric A.Wong;Zehava Uni-.Nutritional stimulation by in-ovo feeding modulates cellular proliferation and differentiation in the small intestinal epithelium of chicks)[J].动物营养（英文）,2022(01):91-101

ovo,Broiler,Multipotent,Lgr5+stem,PepT1+region,mentalization

Nutritional,stimulation,by,feeding,modulates,cellular,proliferation,differentiation,small,intestinal,epithelium,chicks,developing,intestine,embryos,conducted,IOF,We,hypothesized,that,glutamine,leucine,opment,promoting,multipotent,epithelial,cells,were,subject,Gln,Leu,NaCl,injection,control,embryonic,progenitor,differentiated,located,quantified,between,after,hatch,groups,immunofluorescence,SRY,box,transcription,Sox9,proliferating,nuclear,antigen,PCNA,situ,hybridization,rich,repeat,containing,protein,coupled,receptor,peptide,transporter,histochemical,goblet,staining,effects,treatments,post,comparison,follows,total,counts,increased,respectively,Only,exhibited,significance,All,shifted,localizations,villus,bottoms,villi,was,fold,longer,while,densities,decreased,Post,crypt,significantly,higher,compared,conclude,stimulates,maturation,functionality,during,period,resulting,enhanced,compart,niches,expansions,absorptive,surface,area

0.409092