Single-molecule detection and imaging are of great value in chemical analysis,biomarker identification and other trace detection fields.However,the localization and visualization of single molecule are still quite a challenge.Here,we report a special-engineered nanostructure of Ag nanoparticles embedded in multi-layer black phosphorus nanosheets(Ag/BP-NS)synthesized by a unique photoreduction method as a surface-enhanced Raman scattering(SERS)sensor.Such a SERS substrate features the lowest detection limit of 10-20 mol L-1 for R6G,which is due to the three synergistic resonance enhancement of molecular resonance,photo-induced charge transfer resonance and electromagnetic resonance.We pro-pose a polarization-mapping strategy to realize the detection and visualiza-tion of single molecule.In addition,combined with machine learning,Ag/BP-NS substrates are capable of recognition of different tumor exosomes,which is meaningful for monitoring and early warning of the cancer.This work provides a reliable strategy for the detection of single molecule and a potential candidate for the practical bio-application of SERS technology.

Chenglong Lin;Shunshun Liang;Yusi Peng;Li Long;Yanyan Li;Zhengren Huang;Nguyen Viet Long;Xiaoying Luo;Jianjun Liu;Zhiyuan Li;Yong Yang

State Key Laboratory of High-Performance Ceramics and Superfine Microstructures,Shanghai Institute of Ceramics,Chinese Academy of Sciences,1295 Dingxi Road,Shanghai 200050,People's Republic of China;Graduate School of the Chinese Academy of Sciences,No.19(A)Yuquan Road,Beijing 100049,People's Republic of China;Center of Materials Science and Optoelectronics Engineering,University of Chinese Academy of Sciences,Beijing 100049,People's Republic of China;State Key Laboratory of Oncogenes and Related Genes,Shanghai Cancer Institute,Renji Hospital,Shanghai Jiaotong University School of Medicine,200032 Shanghai,People's Republic of China;School of Physics and Optoelectronics,South China University of Technology,Guangzhou 510641,People's Republic of China;Department of Electronics and Telecommunications,Saigon University,Hochiminh City,Vietnam

纳微快报（英文）

[1]Chenglong Lin;Shunshun Liang;Yusi Peng;Li Long;Yanyan Li;Zhengren Huang;Nguyen Viet Long;Xiaoying Luo;Jianjun Liu;Zhiyuan Li;Yong Yang-.Visualized SERS Imaging of Single Molecule by Ag/Black Phosphorus Nanosheets)[J].纳微快报（英文）,2022(05):110-124

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