Objective:To study the effects of total ginsenosides(TG)extract from Panax ginseng on neural stem cell(NSC)proliferation and differentiation and their underlying mechanisms.Methods:The migration of NSCs after treatment with various concentrations of TG extract(50,100,or 200 μg/mL)were monitored.The proliferation of NSCs was examined by a combination of cell counting kit-8 and neurosphere assays.NSC differentiation mediated by TG extract was evaluated by Western blotting and immunofluorescence staining to monitor the expression of nestin and microtubule associated protein 2(MAP2).The GSK-3 β/β-catenin pathway in TG-treated NSCs was examined by Western blot assay.The NSCs with constitutively active GSK-3 βmutant were made by adenovirus-mediated gene transfection,then the proliferation and differentiation of NSCs mediated by TG were further verified.Results:TG treatment significantly enhanced NSC migration(P＜0.01 or P＜0.05)and increased the proliferation of NSCs(P＜0.01 or P＜0.05).TG mediation also significantly upregulated MAP2 expression but downregulated nestin expression(P＜0.01 or P＜0.05).TG extract also significantly induced GSK-3 β phosphorylation at Ser9,leading to GSK-3 β inactivation and,consequently,the activation of the GSK-3 β/β-catenin pathway(P＜0.01 or P＜0.05).In addition,constitutive activation of GSK-3 β in NSCs by the transfection of GSK-3 β S9A mutant was found to significantly suppress TG-mediated NSC proliferation and differentiation(P＜0.01 or P＜0.05).Conclusion:TG promoted NSC proliferation and neuronal differentiation by inactivating GSK-3 β.

LIN Kai-li;ZHANG Ji;CHUNG Hau-lam;WU Xin-yi;LIU Bin;ZHAO Bo-xin;SZE Stephen Cho-wing;ZHOU Ping-zheng;YUNG Ken Kin-lam;ZHANG Shi-qing

School of Public Health,Guangzhou Medical University,Guangzhou 511436,China;Department of Biology,Hong Kong Baptist University(HKBU),Hong Kong Special Administrative Region 999077,China;HKBU Shenzhen Research Institute and Continuing Education,Shenzhen,Guangdong Province 518057,China;Department of Plastic Surgery,Zhujiang Hospital,Southern Medical University,Guangzhou 510515,China;Guangzhou Institute of Cardiovascular Disease,the Second Affiliated Hospital of Guangzhou Medical University,Guangzhou 510260,China;Department of Pharmacy,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China;Guangdong Provincial Key Laboratory of New Drug Screening,School of Pharmaceutical Sciences,Southern Medical University,Guangzhou 510515,China

中国结合医学杂志（英文版）

[1]LIN Kai-li;ZHANG Ji;CHUNG Hau-lam;WU Xin-yi;LIU Bin;ZHAO Bo-xin;SZE Stephen Cho-wing;ZHOU Ping-zheng;YUNG Ken Kin-lam;ZHANG Shi-qing-.Total Ginsenoside Extract from Panax ginseng Enhances Neural Stem Cell Proliferation and Neuronal Differentiation by Inactivating GSK-3β)[J].中国结合医学杂志（英文版）,2022(03):229-235

Inactivating,neurosphere,S9A

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