典型文献
Anti-pseudo-allergic components in licorice extract inhibit mast cell degranulation and calcium influx
文献摘要：
Pseudo-allergic reactions(PARs)widely occur upon application of drugs or functional foods.Anti-pseudo-allergic in-gredients from natural products have attracted much attention.This study aimed to investigate anti-pseudo-allergic compounds in licorice.The anti-pseudo-allergic effect of licorice extract was evaluated in rat basophilic leukemia 2H3(RBL-2H3)cells.Anti-pseudo-allergic compounds were screened by using RBL-2H3 cell extraction and the effects of target components were verified further in RBL-2H3 cells,mouse peritoneal mast cells(MPMCs)and mice.Molecular docking and human MRGPRX2-expressing HEK293T cells(MRGPRX2-HEK293T cells)extraction were performed to determine the potential ligands of MAS-related G protein-coupled receptor-X2(MRGPRX2),a pivotal target for PARs.Glycyrrhizic acid(GA)and licorice chalcone A(LA)were screened and shown to inhibit Compound48/80-induced degranulation and calcium influx in RBL-2H3 cells.GA and LA also inhibited degranulation in MPMCs and increase of histamine and TNF-α in mice.LA could bind to MRGPRX2,as determined by molecular docking and MRGPRX2-HEK293T cell extraction.Our study provides a strong rationale for using GA and LA as novel treatment options for PARs.LA is a po-tential ligand of MRGPRX2.
文献关键词：
中图分类号：
作者姓名：
WANG Lu;HU Gui-Zhou;LU Yin;JIANG Shu-Jun;QI Jin;SU Hua
作者机构：
National Center of Colorectal Disease,Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medi-cine,Nanjing 210022,China;Jiangsu Key Laboratory of TCM Evaluation and Translation Research,School of Traditional Chinese Pharmacy,China Phar-maceutical University,Nanjing 211198,China;Department of Pharmacy,Jinling Hospital,Nanjing 210002,China
文献出处：
引用格式：
[1]WANG Lu;HU Gui-Zhou;LU Yin;JIANG Shu-Jun;QI Jin;SU Hua-.Anti-pseudo-allergic components in licorice extract inhibit mast cell degranulation and calcium influx)[J].中国天然药物,2022(06):421-431
A类：
PARs,gredients,basophilic,MPMCs,MRGPRX2
B类：
Anti,pseudo,allergic,components,licorice,mast,degranulation,calcium,influx,Pseudo,reactions,widely,occur,upon,application,drugs,functional,foods,from,natural,products,have,attracted,much,attention,This,study,aimed,investigate,anti,compounds,was,evaluated,leukemia,2H3,RBL,cells,were,screened,by,using,extraction,effects,target,verified,further,mouse,peritoneal,mice,Molecular,docking,human,expressing,HEK293T,performed,potential,ligands,MAS,related,protein,coupled,receptor,pivotal,Glycyrrhizic,acid,GA,chalcone,LA,shown,Compound48,induced,also,inhibited,increase,histamine,could,bind,determined,molecular,Our,provides,strong,rationale,novel,treatment,options
AB值：
0.42494
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机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
