The wood-feeding termite Coptotermes formosanus represents a unique and impressive system for lignocellulose degradation.The highly efficient digestion of lig-nocellulose is achieved through symbiosis with gut symbionts like bacteria.Despite ex-tensive research during the last three decades,diversity of bacterial symbionts residing in individual gut regions of the termite and their associated functions is still lacking.To this end,cellulose,xylan,and dye-decolorization bacteria residing in foregut,midgut,and hindgut regions of C.formosanus were enlisted by using enrichment and culture-dependent molecular methods.A total of 87 bacterial strains were successfully isolated from different gut regions of C.formosanus which belonged to 27 different species of 10 genera,majorly affiliated with Proteobacteria(80％)and Firmicutes(18.3％).Among the gut regions,37.9％of the total bacterial isolates were observed in the hindgut that demon-strated predominance of cellulolytic bacteria(47.6％).The majority of the xylanolytic and dye-decolorization bacteria(50％)were obtained from the foregut and midgut,respec-tively.Actinobacteria represented by Dietza sp.was observed in the hindgut only.Based on species richness,the highest diversity was observed in midgut and hindgut regions each of which harbored seven unique bacterial species.The members of Enterobacter,Klebsiella,and Pseudomonas were common among the gut regions.The lignocellulolytic activities of the selected potential bacteria signpost their assistance to the host for lignocellulose digestion.The overall results indicate that C.formosanus harbors diverse communities of lignocellulolytic bacteria in different regions of the gut system.These observations will significantly advance our understanding of the termite-bacteria symbiosis and their mi-crobial ecology uniquely existed in different gut regions of C.formosanus,which may further shed a light on its potential values at termite-modeled biotechnology.

Mudasir A.Dar;Rongrong Xie;Radhakrishna S.Pandit;Blessing Danso;Chenchen Dong;Jianzhong Sun

Biofuels Institute,School of the Environment and Safety Engineering,Jiangsu University,Zhenjiang,Jiangsu Province,China;Department of Zoology,Savitribai Phule Pune University,Ganeshkhind,Pune,India

中国昆虫科学（英文版）

[1]Mudasir A.Dar;Rongrong Xie;Radhakrishna S.Pandit;Blessing Danso;Chenchen Dong;Jianzhong Sun-.Exploring the region-wise diversity and functions of symbiotic bacteria in the gut system of wood-feeding termite,Coptotermes formosanus,toward the degradation of cellulose,hemicellulose,and organic dyes)[J].中国昆虫科学（英文版）,2022(05):1414-1432

nocellulose,enlisted,cellulolytic,xylanolytic,Dietza,lignocellulolytic,signpost

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