典型文献
Identification of two ABCC transporters involved in malathion detoxification in the red flour beetle,Tribolium castaneum
文献摘要：
ABC transporters have been suggested to be involved in insecticide detoxifica-tion in different insect species mainly based on the indirect observation of transcriptional upregulation of ABC gene expression in response to insecticide exposure.Previous studies performed by us and others in the red flour beetle,Tribolium castaneum,have analyzed the function of TcABCA-C and TcABCG-H genes using RNA interference(RNAi)and demonstrated that specific TcABCA and TcABCC genes are involved in the elimination of the pyrethroid tefluthrin and the benzoylurea diflubenzuron,because gene silencing increased the beetle's susceptibility to the insecticides.In this study,we focused on the po-tential functions of TcABCA-C genes in detoxification of the pyrethroid cyfluthrin(CF),the organophosphate malathion(MAL)and the diacylhdyazine tebufenozide(TBF).Anal-ysis of transcript levels of selected TcABCA-C genes in response to treatment with these three chemically unrelated insecticides revealed that some genes were particularly upreg-ulated after insecticide treatment.In addition,the ABC inhibitor verapamil synergized significantly the toxicity of MAL but only negligibly CF and TBF toxicities.Finally,si-lencing of two TcABCC genes by RNAi revealed a significant increase in susceptibility to MAL.In contrast,we did not observe a significant increase in insecticide-induced mor-talities when knocking down TcABC genes in larvae treated with CF or TBF,although they were upregulated in response to insecticide treatment.Our results suggest that two pleiotropic ABCC transporters expressed in metabolic and excretory tissues contribute to the elimination of MAL.
文献关键词：
中图分类号：
作者姓名：
Janin R?sner;Hans Merzendorfer
作者机构：
Department of Chemistry-Biology,Institute of Biology,University of Siegen,Siegen,Germany
文献出处：
引用格式：
[1]Janin R?sner;Hans Merzendorfer-.Identification of two ABCC transporters involved in malathion detoxification in the red flour beetle,Tribolium castaneum)[J].中国昆虫科学（英文版）,2022(04):1096-1104
A类：
malathion,detoxifica,TcABCA,TcABCG,TcABCC,tefluthrin,benzoylurea,diflubenzuron,cyfluthrin,diacylhdyazine,tebufenozide,upreg,lencing,talities,TcABC
B类：
Identification,two,transporters,involved,detoxification,red,flour,beetle,Tribolium,castaneum,have,been,suggested,different,species,mainly,indirect,observation,transcriptional,upregulation,expression,response,exposure,Previous,studies,performed,by,others,analyzed,genes,using,interference,RNAi,demonstrated,that,specific,are,elimination,pyrethroid,because,silencing,increased,susceptibility,insecticides,In,this,study,focused,tential,functions,CF,organophosphate,MAL,TBF,Anal,ysis,levels,selected,treatment,these,three,chemically,unrelated,revealed,some,were,particularly,after,addition,inhibitor,verapamil,synergized,significantly,toxicity,only,negligibly,toxicities,Finally,contrast,did,not,observe,induced,mor,when,knocking,down,larvae,treated,although,they,upregulated,Our,results,pleiotropic,expressed,metabolic,excretory,tissues,contribute
AB值：
0.418256
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