典型文献
Comparative analyses of the venom components in the salivary gland transcriptomes and saliva proteomes of some heteropteran insects
文献摘要：
Salivary gland-specific transcriptomes of nine heteropteran insects with distinct feeding strategies(predaceous,hematophagous,and phytophagous)were analyzed and annotated to compare and identify the venom components as well as their expres-sion profiles.The transcriptional abundance of venom genes was verified via quantita-tive real-time PCR.Hierarchical clustering of 30 representative differentially expressed venom genes from the nine heteropteran species revealed unique groups of salivary gland-specific genes depending on their feeding strategy.The commonly transcribed genes in-cluded a paralytic neurotoxin(arginine kinase),digestive enzymes(cathepsin and serine protease),an anti-inflammatory protein(cystatin),hexamerin,and an odorant binding pro-tein.Both predaceous and hematophagous(bed bug)heteropteran species showed rela-tively higher transcription levels of genes encoding proteins involved in proteolysis and cytolysis,whereas phytophagous heteropterans exhibited little or no expression of these genes,but had a high expression of vitellogenin,a multifunctional allergen.Saliva pro-teomes from four representative species were also analyzed.All venom proteins iden-tified via saliva proteome analysis were annotated using salivary gland transcriptome data.The proteomic expression profiles of venom proteins were in good agreement with the salivary gland-specific transcriptomic profiles.Our results indicate that profiling of the salivary gland transcriptome provides important information on the composition and evolutionary features of venoms depending on their feeding strategy.
文献关键词：
中图分类号：
作者姓名：
Kyungjae Andrew Yoon;Woo-Jin Kim;Seungki Lee;Hee-Sun Yang;Byoung-Hee Lee;Si Hyeock Lee
作者机构：
Research Institute of Agriculture and Life Sciences,Seoul National University,Seoul,Korea;EntoCode Co.,Seoul,Korea;National Institute of Biological Resources,Environmental Research Complex,Incheon,Korea;Department of Agricultural Biology,Seoul National University,Seoul,Korea
文献出处：
引用格式：
[1]Kyungjae Andrew Yoon;Woo-Jin Kim;Seungki Lee;Hee-Sun Yang;Byoung-Hee Lee;Si Hyeock Lee-.Comparative analyses of the venom components in the salivary gland transcriptomes and saliva proteomes of some heteropteran insects)[J].中国昆虫科学（英文版）,2022(02):411-429
A类：
heteropteran,Salivary,predaceous,hematophagous,phytophagous,hexamerin,cytolysis,heteropterans,teomes,venoms
B类：
Comparative,analyses,components,salivary,gland,transcriptomes,proteomes,some,insects,specific,distinct,feeding,strategies,were,analyzed,annotated,compare,identify,well,their,profiles,transcriptional,abundance,genes,was,verified,via,quantita,real,Hierarchical,clustering,representative,differentially,expressed,from,species,revealed,unique,groups,depending,strategy,commonly,transcribed,cluded,paralytic,neurotoxin,arginine,kinase,digestive,enzymes,cathepsin,serine,protease,inflammatory,cystatin,odorant,binding,Both,bug,showed,rela,tively,higher,levels,encoding,proteins,involved,proteolysis,whereas,exhibited,little,expression,these,but,had,vitellogenin,multifunctional,allergen,four,also,All,tified,analysis,using,data,proteomic,good,agreement,transcriptomic,Our,results,indicate,that,profiling,provides,important,information,composition,evolutionary,features
AB值：
0.449377
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