典型文献
Molecular and functional characterization of three novel carboxylesterases in the detoxification of permethrin in the mosquito,Culex quinquefasciatus
文献摘要：
Carboxylesterases (CarEs) belong to a super family of multifunctional en-zymes associated with the degradation of endogenous and exogenous compounds.Many insect CarEs are known to play important roles in catalyzing the hydrolysis of organophos-phates (OPs),carbamates,and synthetic pyrethroids (SPs).The elevation ofesterase activ-ity through gene amplification and overexpression of estα2 and estβ2 genes contributes to the development of resistance to OP insecticides in the mosquito Culex quinquefasciatus.Three additional CarE genes are upregulated in permethrin-resistant Cx.quinquefascia-tus according to an RNA-seq analysis,but their function remains unknown.In this study,we,for the first time,characterized the function of these three novel genes using in vitro protein expression,an insecticide metabolism study and molecular docking analysis.All three CarE genes were significantly overexpressed in resistant mosquito larvae,but not adults,compared to susceptible strain.No gene copy differences in these three genes were found in the mosquitoes tested.In vitro high-performance liquid chromatography (HPLC)revealed that CPIJ018231,CPIJ018232,and CPIJ018233 metabolized 30.4％ ± 2.9％,34.7％ ± 6.8％,and 23.2％ ± 2.2％ of the permethrin,respectively.No mutations in re-sistant strains might significantly affect their CarE hydrolysis ability.A docking analysis further confirmed that these three CarEs from resistant strain all potentially metabolize permethrin.Taken together,these three carboxylesterase genes could play important roles in the development of permethrin resistance in Cx.quinquefasciatus larvae through tran-scriptional overexpression,metabolism,and detoxification.
文献关键词：
中图分类号：
作者姓名：
Youhui Gong;Ming Li;Ting Li;Nannan Liu
作者机构：
Department of Entomology and Plant Pathology,Auburn University,Auburn,Alabama,USA;State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing,China;Department of Biology Sciences,University of California,San Diego,California,USA
文献出处：
引用格式：
[1]Youhui Gong;Ming Li;Ting Li;Nannan Liu-.Molecular and functional characterization of three novel carboxylesterases in the detoxification of permethrin in the mosquito,Culex quinquefasciatus)[J].中国昆虫科学（英文版）,2022(01):199-214
A类：
carboxylesterases,permethrin,quinquefasciatus,Carboxylesterases,CarEs,organophos,phates,carbamates,ofesterase,quinquefascia,CPIJ018231,CPIJ018232,CPIJ018233
B类：
Molecular,characterization,three,novel,detoxification,Culex,belong,super,family,multifunctional,zymes,associated,degradation,endogenous,exogenous,compounds,Many,play,important,roles,catalyzing,hydrolysis,OPs,synthetic,pyrethroids,SPs,elevation,activ,through,amplification,overexpression,genes,contributes,development,resistance,insecticides,Three,additional,upregulated,resistant,Cx,according,seq,analysis,their,remains,unknown,In,this,study,first,characterized,these,using,vitro,protein,metabolism,molecular,docking,All,were,significantly,overexpressed,larvae,not,adults,compared,susceptible,No,copy,differences,found,mosquitoes,tested,high,performance,liquid,chromatography,HPLC,revealed,that,metabolized,respectively,mutations,strains,might,affect,ability,further,confirmed,from,potentially,Taken,together,could,tran,scriptional
AB值：
0.412451
相似文献
机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
