Juvenile hormone (JH) absence induces photoperiod-mediated reproduc-tive diapause,which is characterized by reproductive cessation.Although the role of methoprene-tolerant (Met)-mediated JH signaling in photoperiod-mediated female repro-duction has been well documented,its role in male reproduction remains unclear.In this study,we investigated the role of JH in regulating photoperiod-mediated development of the male internal reproductive system (IRS) in the predatory ladybeetle Harmonia axyridis(Pallas).In a previous study,we found that adult male H.axyridis reared under either a short-day (SD) or long-day (LD) photoperiod had obvious differences in IRS development,but we were unable to identify the regulators of male reproductive diapause.In this study,we found that beetles reared under an SD photoperiod had significantly lower JH titer and a relatively undeveloped male IRS compared with those reared under an LD photoperiod.Additionally,application of the JH analog (JHA) methoprene promoted IRS development.Furthermore,Met knockdown strongly blocked JH signaling in males reared under the LD photoperiod,thereby slowing IRS development.Moreover,exogenous JHA did not reverse the suppressed development of the male IRS caused by Met knockdown.These results indicate that photoperiod regulates male IRS development in H.axyridis through a conserved Met-dependent JH signaling pathway.

Qiao Gao;Bei Li;Bina-Xin Wei;Wen Liu;Pan Wang;Jia-Lu Wang;Xing-Miao Zhou;Xiao-Ping Wang

Hubei Key Laboratory of Insect Resources Utilization and Sustainable Pest Management,College of Plant Science and Technology,Huazhong Agricultural University,Wuhan,430070,China;Department of Plant Protection,Wuhan Vegetable Research Institute,Wuhan,430065,China

中国昆虫科学（英文版）

[1]Qiao Gao;Bei Li;Bina-Xin Wei;Wen Liu;Pan Wang;Jia-Lu Wang;Xing-Miao Zhou;Xiao-Ping Wang-.Juvenile hormone regulates photoperiod-mediated male reproductive diapause via the methoprene-tolerant gene in the ladybeetle Harmonia axyridis)[J].中国昆虫科学（英文版）,2022(01):139-150

ladybeetle

Juvenile,hormone,regulates,photoperiod,mediated,reproductive,diapause,via,methoprene,tolerant,gene,Harmonia,axyridis,absence,induces,which,characterized,cessation,Although,role,Met,signaling,female,has,been,well,documented,its,reproduction,remains,unclear,In,this,study,investigated,regulating,development,internal,system,IRS,predatory,Pallas,previous,found,that,adult,reared,under,either,short,day,long,LD,had,obvious,differences,but,were,unable,identify,regulators,beetles,significantly,lower,titer,relatively,undeveloped,compared,those,Additionally,application,analog,JHA,promoted,Furthermore,knockdown,strongly,blocked,males,thereby,slowing,Moreover,exogenous,did,not,reverse,suppressed,caused,These,results,indicate,through,conserved,dependent,pathway

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