Subgroup J avian leukosis virus(ALV-J)is a highly oncogenic retrovirus that has been devastating the global poultry industry since the late 1990s.The major infection model of ALV-J is vertical transmission,which is responsible for the congenital infection of progeny from generation to generation.Increasing evidence has sug-gested that extracellular vesicles(EVs)derived from virus-infected cells or biological fluids have been thought to be vehicles of transmission for viruses.However,the role of EVs in infection and transmission of ALV-J remains obscure.In the present study,semen extracellular vesicles(SE)were isolated and purified from ALV-J-infected rooster seminal plasma(SE-ALV-J),which was shown to contain ALV-J genomic RNA and partial viral pro-teins,as determined by RNA sequencing,reverse transcription-quantitative PCR and Western blotting.Further-more,SE-ALV-J was proved to be able to transmit ALV-J infection to host cells and establish productive infection.More importantly,artificial insemination experiments showed that SE-ALV-J transmitted ALV-J infection to SPF hens,and subsequently mediated vertical transmission of ALV-J from the SPF hens to the progeny chicks.Taken together,the results of the present study suggested that ALV-J utilized host semen extracellular vesicles as a novel means for vertical transmission,enhancing our understanding on mechanisms underlying ALV-J transmission.

Liqin Liao;Weiguo Chen;Xiangyu Zhang;Huanmin Zhang;Aijun Li;Yiming Yan;Zi Xie;Hongxing Li;Wencheng Lin;Jingyun Ma;Xinheng Zhang;Qingmei Xie

Heyuan Branch,Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology,College of Animal Science,South China Agricultural University,Guangzhou,510642,China;Guangdong Provincial Key Lab of Agro Animal Genomics and Molecular Breeding,Guangzhou,510642,China;Key Laboratory of Animal Health Aquaculture and Environmental Control,Guangdong,Guangzhou,510642,China;Guangdong Engineering Research Center for Vector Vaccine of Animal Virus,Guangzhou,510642,China;USDA,Agriculture Research Service,Avian Disease and Oncology Laboratory,East Lansing,MI,48823,USA;College of Science and Engineering,Jinan University,Guangzhou,510632,China

中国病毒学

[1]Liqin Liao;Weiguo Chen;Xiangyu Zhang;Huanmin Zhang;Aijun Li;Yiming Yan;Zi Xie;Hongxing Li;Wencheng Lin;Jingyun Ma;Xinheng Zhang;Qingmei Xie-.Semen extracellular vesicles mediate vertical transmission of subgroup J avian leukosis virus)[J].中国病毒学,2022(02):284-294

rooster

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