典型文献
Nucleolin interacts with the rabbit hemorrhagic disease virus replicase RdRp,nonstructural proteins p16 and p23,playing a role in virus replication
文献摘要：
Rabbit hemorrhagic disease virus(RHDV)is a member of the Caliciviridae family and cannot be propagated in vitro,which has impeded the progress of investigating its replication mechanism.Construction of an RHDV replicon system has recently provided a platform for exploring RHDV replication in host cells.Here,aided by this replicon system and using two-step affinity purification,we purified the RHDV replicase and identified its associated host factors.We identified rabbit nucleolin(NCL)as a physical link,which mediating the interaction between other RNA-dependent RNA polymerase(RdRp)-related host proteins and the viral replicase RdRp.We found that the overexpression or knockdown of NCL significantly increased or severely impaired RHDV repli-cation in RK-13 cells,respectively.NCL was identified to directly interact with RHDV RdRp,p16,and p23.Furthermore,NCL knockdown severely impaired the binding of RdRp to RdRp-related host factors.Collectively,these results indicate that the host protein NCL is essential for RHDV replication and acts as a physical link be-tween viral replicase and host proteins.
文献关键词：
中图分类号：
作者姓名：
Jie Zhu;Qiuhong Miao;Hongyuan Guo;Aoxing Tang;Dandan Dong;Jingyu Tang;Fang Wang;Guangzhi Tong;Guangqing Liu
作者机构：
Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai,200241,China;Laboratory of Virology,Wageningen University and Research,Wageningen,6708 PB,the Netherlands;Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences,Nanjing,210014,China
文献出处：
引用格式：
[1]Jie Zhu;Qiuhong Miao;Hongyuan Guo;Aoxing Tang;Dandan Dong;Jingyu Tang;Fang Wang;Guangzhi Tong;Guangqing Liu-.Nucleolin interacts with the rabbit hemorrhagic disease virus replicase RdRp,nonstructural proteins p16 and p23,playing a role in virus replication)[J].中国病毒学,2022(01):48-59
A类：
Nucleolin,replicase,Caliciviridae,replicon,nucleolin
B类：
interacts,rabbit,hemorrhagic,disease,virus,RdRp,nonstructural,proteins,p16,p23,playing,role,replication,Rabbit,RHDV,member,family,cannot,propagated,vitro,which,has,impeded,progress,investigating,its,mechanism,Construction,system,recently,provided,platform,exploring,host,cells,Here,aided,by,this,using,two,step,affinity,purification,purified,identified,associated,factors,We,NCL,physical,link,mediating,interaction,between,other,dependent,polymerase,related,viral,found,that,overexpression,knockdown,significantly,increased,severely,impaired,RK,respectively,was,directly,Furthermore,binding,Collectively,these,results,indicate,essential
AB值：
0.432058
相似文献
机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
