Prostate cancer(PCa)is one of the most frequent cancers in men,and its biomolecular targets have been extensively studied.This study aimed to analyze the expression of toll-like receptor 9(TLR9)and vascular endothelial growth factor C(VEGF-C)and the clinical value of the coexpression of TLR9 and VEGF-C in PCa.We retrospectively evaluated 55 patients with clinically localized,intermediate-risk,or high-risk PCa who underwent laparoscopic radical prostatectomy(LRP)and extended pelvic lymph node dissection(ePLND)without neoadjuvant hormonal therapy at a single institution from June 2013 to December 2016.In all 55 patients,the median number of lymph nodes(LNs)resected was 23(range:18-31),and a total of 1269 LNs were removed,of which 78 LNs were positive.Seventeen patients had positive LNs,with a positive rate of 30.9％.In addition,the immunohistochemical results in the above patients revealed that high TLR9 expression was correlated with higher Gleason score(GS)(P=0.049),increased LN metastasis(P=0.004),and more perineural invasion(PNI)(P=0.033).Moreover,VEGF-C expression was associated with GS(P=0.040),pathological stage(pT stage)(P=0.022),LN metastasis(P=0.003),and PNI(P=0.001).Furthermore,a significant positive correlation between TLR9 and VEGF-C was found(P＜0.001),and the TLR9/VEGF-C phenotype was associated with LN metastasis(P=0.047).Collectively,we propose that TLR9 stimulation may promote LN metastasis in PCa cells through the upregulation of VEGF-C expression,thereby affecting the prognosis of PCa patients.Therefore,these markers may serve as valuable targets for the treatment of PCa.

Xian-Zi Zeng;Zhan-Sen Huang;Hong-Peng Fang;Jie-Ying Wu;Qun-Xiong Huang;Chu-Bin Zhuang;Jing Zhou;Jin-Ming Di

Department of Urology,The Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510630,China;Department of Infertility and Sexual Medicine,The Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510630,China;Department of Pathology,The Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510630,China

亚洲男性学杂志（英文版）

[1]Xian-Zi Zeng;Zhan-Sen Huang;Hong-Peng Fang;Jie-Ying Wu;Qun-Xiong Huang;Chu-Bin Zhuang;Jing Zhou;Jin-Ming Di-.Coexpression of TLR9 and VEGF-C is associated with lymphatic metastasis in prostate cancer)[J].亚洲男性学杂志（英文版）,2022(04):380-385

Coexpression,ePLND

TLR9,VEGF,associated,lymphatic,metastasis,Prostate,PCa,one,most,frequent,cancers,its,biomolecular,targets,have,been,extensively,studied,This,study,aimed,analyze,toll,like,receptor,vascular,endothelial,growth,value,coexpression,We,retrospectively,evaluated,patients,clinically,localized,intermediate,risk,who,underwent,laparoscopic,radical,prostatectomy,LRP,extended,pelvic,dissection,without,neoadjuvant,hormonal,therapy,single,institution,from,June,December,In,median,number,nodes,LNs,resected,was,range,total,were,removed,which,positive,Seventeen,had,rate,addition,immunohistochemical,results,above,revealed,that,correlated,higher,Gleason,score,GS,increased,perineural,invasion,PNI,Moreover,pathological,stage,pT,Furthermore,significant,correlation,between,found,phenotype,Collectively,propose,stimulation,may,promote,cells,through,upregulation,thereby,affecting,prognosis,Therefore,these,markers,serve,valuable,treatment

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