Abstinence from prolonged psychostimulant use prompts stimulant withdrawal syndrome.Molecular adaptations within the dorsal striatum have been considered the main hallmark of stimulant abstinence.Here we explored striatal miRNA-target interaction and its impact on circulating miRNA marker as well as behavioral dysfunctions in methamphetamine(MA)abstinence.We conducted miRNA sequencing and profiling in the nonhuman primate model of MA abstinence,followed by miRNA qPCR,LC-MS/MS proteomics,immunoassays,and behavior tests in mice.In nonhuman primates,MA absti-nence triggered a lasting upregulation of miR-137 in the dorsal striatum but a simultaneous downregula-tion of circulating miR-137.In mice,aberrant increase in striatal miR-137-dependent inhibition of SYNCRIP essentially mediated the MA abstinence-induced reduction of circulating miR-137.Pathway modeling through experimental deduction illustrated that the MA abstinence-mediated downregulation of circulating miR-137 was caused by reduction of SYNCRIP-dependent miRNA sorting into the exosomes in the dorsal striatum.Furthermore,diminished SYNCRIP in the dorsal striatum was necessary for MA abstinence-induced behavioral bias towards egocentric spatial learning.Taken together,our data revealed circulating miR-137 as a potential blood-based marker that could reflect MA abstinence-dependent changes in striatal miR-137/SYNCRIP axis,and striatal SYNCRIP as a potential therapeutic target for striatum-associated cognitive dysfunction by MA withdrawal syndrome.

Baeksun Kim;Sung Hyun Tag;Eunjoo Nam;Suji Ham;Sujin Ahn;Juhwan Kim;Doo-Wan Cho;Sangjoon Lee;Young-Su Yang;Seung Eun Lee;Yong Sik Kim;Il-Joo Cho;Kwang Pyo Kim;Su-Cheol Han;Heh-In Im

Convergence Research Center for Diagnosis,Treatment and Care System of Dementia(DTC),Korea Institute of Science and Technology(KIST),Seoul 02792,Republic of Korea;Division of Bio-Medical Science&Technology,KIST School,Korea University of Science and Technology(UST),Seoul 02792,Republic of Korea;Center for Neuroscience,KIST Seoul 02792,Republic of Korea;Animal Model Research Center Jeonbuk Department of Inhalation Research,Korea Institute of Toxicology(KIT),Korea Research Institute of Chemical Technology(KRICT),Jeongeup 56212,Republic of Korea;Research Animal Resource Center KIST Seoul 02792,Republic of Korea;Department of Pharmacology,Seoul National University College of Medicine,Seoul 03080,Republic of Korea;Center for BioMicrosystems,KIST Seoul 02792,Republic of Korea;Department of Applied Chemistry,Kyung Hee University,Gyeonggi-do 17104,Republic of Korea

药学学报（英文版）

[1]Baeksun Kim;Sung Hyun Tag;Eunjoo Nam;Suji Ham;Sujin Ahn;Juhwan Kim;Doo-Wan Cho;Sangjoon Lee;Young-Su Yang;Seung Eun Lee;Yong Sik Kim;Il-Joo Cho;Kwang Pyo Kim;Su-Cheol Han;Heh-In Im-.SYNCRIP controls miR-137 and striatal learning in animal models of methamphetamine abstinence)[J].药学学报（英文版）,2022(08):3281-3297

SYNCRIP,Abstinence,psychostimulant,absti,nence,downregula,egocentric

controls,striatal,learning,animal,models,methamphetamine,abstinence,from,prolonged,prompts,withdrawal,syndrome,Molecular,adaptations,within,dorsal,striatum,have,been,considered,main,hallmark,Here,explored,miRNA,target,interaction,its,impact,circulating,marker,well,behavioral,dysfunctions,MA,We,conducted,sequencing,profiling,nonhuman,followed,by,qPCR,LC,proteomics,immunoassays,tests,mice,In,primates,triggered,lasting,upregulation,but,simultaneous,aberrant,increase,dependent,inhibition,essentially,mediated,induced,reduction,Pathway,modeling,through,experimental,deduction,illustrated,that,downregulation,was,caused,sorting,into,exosomes,Furthermore,diminished,necessary,bias,towards,spatial,Taken,together,our,data,revealed,potential,blood,could,reflect,changes,axis,therapeutic,associated,cognitive

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