典型文献
Structural insights into the membrane microdomain organization by SPFH family proteins
文献摘要：
The lateral segregation of membrane constituents into functional microdomains,conceptually known as lipid raft,is a universal organization principle for cellular membranes in both prokaryotes and eukaryotes.The widespread (S)tomatin,(P)rohibitin,Flotillin,and HflK/C (SPFH)family proteins are enriched in functional membrane microdomains at various subcellular locations,and therefore were hypothesized to play a scaffolding role in mlcrodomain formation.In addition,many SPFH proteins are also implicated in highly specific processes occurring on the membrane.However,none of these functions is understood at the molecular level.Here we report the structure of a supramolecular complex that is isolated from bacterial membrane microdomains and contains two SPFH proteins (HflK and HflC) and a membrane-anchored AAA+ protease FtsH.HflK and HflC form a circular 24-mer assembly,featuring a laterally segregated membrane microdomain (20 nm in diameter) bordered by transmembrane domains of HflK/C and a completely sealed periplasmic vault.Four FtsH hexamers are embedded inside this microdomain through interactions with the inner surface of the vault.These observations provide a mechanistic explanation for the role of HflK/C and their mitochondrial homologs prohibitins in regulating membrane-bound AAA+ proteases,and suggest a general model for the organization and functionalization of membrane microdomains by SPFH proteins.
文献关键词：
中图分类号：
作者姓名：
Chengying Ma;Chengkun Wang;Dingyi Luo;Lu Yan;Wenxian Yang;Ningning Li;Ning Gao
作者机构：
State Key Laboratory of Membrane Biology,Peking-Tsinghua Joint Centre for Life Sciences,School of Life Sciences,Peking University,Beijing,China;National Biomedical Imaging Center,Peking University,Beijing,China
文献出处：
引用格式：
[1]Chengying Ma;Chengkun Wang;Dingyi Luo;Lu Yan;Wenxian Yang;Ningning Li;Ning Gao-.Structural insights into the membrane microdomain organization by SPFH family proteins)[J].细胞研究（英文版）,2022(02):176-189
A类：
microdomain,SPFH,tomatin,rohibitin,Flotillin,HflK,mlcrodomain,HflC,AAA+,FtsH,bordered,periplasmic,hexamers,prohibitins
B类：
Structural,insights,into,organization,by,family,proteins,segregation,constituents,microdomains,conceptually,known,lipid,raft,universal,principle,membranes,both,prokaryotes,eukaryotes,widespread,are,enriched,various,subcellular,locations,therefore,were,hypothesized,play,scaffolding,role,formation,In,addition,many,also,implicated,highly,specific,processes,occurring,However,none,these,functions,understood,level,Here,report,structure,supramolecular,complex,that,isolated,from,bacterial,contains,two,anchored,circular,assembly,featuring,laterally,segregated,diameter,transmembrane,completely,sealed,vault,Four,embedded,inside,this,through,interactions,inner,surface,These,observations,provide,mechanistic,explanation,their,mitochondrial,homologs,regulating,bound,proteases,suggest,general,model,functionalization
AB值：
0.468272
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