典型文献
Cycloheximide promotes type Ⅰ collagen maturation mainly via collagen prolyl 4-hydroxylase subunit α2
文献摘要：
Aberrant deposition of collagen is associated with cancer development and tissue fibrosis.Proline hydroxylation,catalyzed by collagen prolyl 4-hydroxylases(C-P4Hs),is necessary for collagen maturation and secretion.Here,we try to evaluate the mechanism of the regulation of CHX on collagen maturation.Using pepsin digestion,liquid chromatograph mass spectrometry and gene knockout,we find that treatment of mouse embryonic fibroblasts with cycloheximide(CHX)increases type Ⅰ collagen proline hydroxylation partially via P4HA1 and mainly via P4HA2.Western blot analysis results show that CHX treatment reduces type Ⅰ collagen but does not obviously impact the level of P4HA1/2 protein in the endoplasmic reticulum,which enhances the molar ratio of P4HA1/2 to type Ⅰ collagen,and coimmunoprecipitation results confirm that more P4HA1/2 can bind to each type Ⅰ collagen.Since C-P4Hs possess the capability to hydroxylate proline independent of ascorbate for a few cycles,this enhanced binding between P4HA1/2 and type Ⅰ collagen can partially explain how CHX stimulates type Ⅰ collagen maturation.
文献关键词：
中图分类号：
作者姓名：
Run Shi;Miao Xu;Huazhe Ye;Shanshan Gao;Jingfeng Li;Huan Li;Chaoyang Li
作者机构：
Affiliated Cancer Hospital&Institute of Guangzhou Medical University,State Key Laboratory of Respiratory Disease,Guangzhou 510095,China
文献出处：
引用格式：
[1]Run Shi;Miao Xu;Huazhe Ye;Shanshan Gao;Jingfeng Li;Huan Li;Chaoyang Li-.Cycloheximide promotes type Ⅰ collagen maturation mainly via collagen prolyl 4-hydroxylase subunit α2)[J].生物化学与生物物理学报（英文版）,2022(12):1832-1840
A类：
hydroxylases,P4Hs,P4HA1,hydroxylate
B类：
Cycloheximide,promotes,type,collagen,maturation,mainly,via,prolyl,subunit,Aberrant,deposition,associated,cancer,development,tissue,fibrosis,Proline,hydroxylation,catalyzed,by,necessary,secretion,Here,evaluate,mechanism,regulation,CHX,Using,pepsin,digestion,liquid,chromatograph,mass,spectrometry,gene,knockout,find,that,treatment,mouse,embryonic,fibroblasts,cycloheximide,increases,proline,partially,P4HA2,blot,analysis,results,show,reduces,but,does,not,obviously,impact,level,protein,endoplasmic,reticulum,which,enhances,molar,coimmunoprecipitation,confirm,more,each,Since,possess,capability,independent,ascorbate,few,cycles,this,enhanced,binding,between,explain,stimulates
AB值：
0.528818
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机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
