Background: Component compatibility is important to the modernization of traditional Chinese medicine. Studies have shown that San?ao decoction (SAD) can treat respiratory diseases by relaxing airway smooth muscle (ASM) and reducing airway hyper?responsiveness. However, whether its bioactive components and compatibility also present with similar relaxant effects remains unknown. This study aims to explore the potential relaxant property, dose-response relationship, and underlying mechanisms of the bioactive component compatibility in SAD. Methods: Network pharmacology was primarily used to identify the bioactive components of SAD and uncover its underlying mechanisms. ASM tension force measuring technique was utilized to verify the relaxant and dose-response effects on in vitro guinea pig ASM. Results: We postulated pseudoephedrine hydrochloride (PH), amygdalin (AM), and diammonium glycyrrhizate (DG) to be the bioactive components of SAD, which could effectively relax ASM in a dose?dependent manner on both acetylcholine?induced and spontaneous contraction. Both PH and AM could lead to DG dose–response curve shift. The regression equation of these three bioactive components was Y = ?2.048 × X1 + 0.411 × X2 + 14.052 × X3 (X1, X2, X3 representing PH, AM, and DG, respectively). The underlying mechanisms of these components might be associated with the regulation of smooth muscle contraction. Conclusions: PH, AM, and DG are the bioactive components of SAD, which can relax ASM in a dose–response manner and exert a synergistic effect. Clinically, compatibility of these three bioactive components may serve as a new complementary and alternative treatment for respiratory diseases.

Wen?Jie Song;Yan?Ling Fu;Sheng?Lou Ni;Jia?Jia Fan;Qian Du;Hao Zheng

Chinese Medical College,School of Traditional Chinese Medicine,Tianjin University of Traditional Chinese Medicine,Jing Hai District,Tianjin,China;Chinese Medical College,School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Chaoyang District,Beijing,China;Periodicals Publishing Center,Beijing University of Chinese Medicine,Chaoyang District,Beijing,China;Wenzhou Traditional Chinese Medicine Hospital,Wenzhou,China

世界中医药杂志（英文版）

[1]Wen?Jie Song;Yan?Ling Fu;Sheng?Lou Ni;Jia?Jia Fan;Qian Du;Hao Zheng-.Relaxant Effect of Bioactive Component Compatibility of San?ao Decoction on In vitro Guinea Pig Airway Smooth Muscle: A Dose-Response Relationship Study)[J].世界中医药杂志（英文版）,2022(02):262-272

Relaxant,relaxant,guinea,pseudoephedrine,glycyrrhizate

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