Bisecting N-acetylglucosamine(GlcNAc),a GlcNAc linked to the core β-mannose resi-due via a β1,4 linkage,is a special type of N-glycosylation that has been reported to be involved in various biological processes,such as cell adhesion and fetal development.This N-glycan structure is abundant in human trophoblasts,which is postulated to be resistant to natural killer cell-mediated cytotoxicity,enabling a mother to nourish a fetus without rejection.In this study,we hypothesized that the human amniotic membrane,which serves as the last barrier for the fetus,may also express bisected-type glycans.To test this hypothesis,glycomic analysis of the human amniotic membrane was performed,and bisected N-glycans were detected.Furthermore,our pro-teomic data,which have been previously employed to explore human missing proteins,were ana-lyzed and the presence of bisecting GlcNAc-modified peptides was confirmed.A total of 41 glycoproteins with 43 glycopeptides were found to possess a bisecting GlcNAc,and 25 of these gly-coproteins were reported to exhibit this type of modification for the first time.These results provide insights into the potential roles of bisecting GlcNAc modification in the human amniotic membrane,and can be beneficial to functional studies on glycoproteins with bisecting GlcNAc modifications and functional studies on immune suppression in human placenta.

Qiushi Chen;Yuanliang Zhang;Keren Zhang;Jie Liu;Huozhen Pan;Xinran Wang;Siqi Li;Dandan Hu;Zhilong Lin;Yun Zhao;Guixue Hou;Feng Guan;Hong Li;Siqi Liu;Yan Ren

BGI-Shenzhen,Shenzhen 518083,China;Joint International Research Laboratory of Glycobiology and Medical Chemistry,College of Life Sciences,Northwest University,Xi,an 710069,China;Shenzhen Seventh People's Hospital,Shenzhen 518081,China;Institute of Interdisciplinary Integrative Medicine Research,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China

基因组蛋白质组与生物信息学报（英文版）

[1]Qiushi Chen;Yuanliang Zhang;Keren Zhang;Jie Liu;Huozhen Pan;Xinran Wang;Siqi Li;Dandan Hu;Zhilong Lin;Yun Zhao;Guixue Hou;Feng Guan;Hong Li;Siqi Liu;Yan Ren-.Profiling the Bisecting N-acetylglucosamine Modification in Amniotic Membrane via Mass Spectrometry)[J].基因组蛋白质组与生物信息学报（英文版）,2022(04):648-656

Bisecting,Amniotic,nourish,bisected,glycomic,teomic,coproteins

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