典型文献
INRA82 extender enhances semen quality in ram under cooled and cryopreserved stages
文献摘要：
Objective: To investigate the effect of INRA82 extender on ram semen quality preserved in cooled and frozen conditions in comparison with Tris-based extenders and to select the suitable extender for ram semen preservation. Methods: Semen samples were collected and pooled from Awassi rams (n=5) and divided into three aliquots. Each aliquot was diluted with one of the following extenders: 1) Tris-citrate glucose, 2) Tris-citrate fructose, and 3) INRA-82. For liquid storage, diluted samples were kept at 5 ℃ for 72 h. Progressive motility was measured at 0, 24, 48 and 72 h after cooling. Besides, viability, morphology, membrane, acrosome and DNA integrities were evaluated at 24 h post-cooling. For cryopreservation, diluted samples were cooled, equilibrated, loaded and frozen in liquid nitrogen. Thawed samples were evaluated in the same manner as cooled conditions. Results: Seminal characteristics were improved after cooling and thawing in samples diluted with INRA-82 compared to those diluted with Tris-citrate glucose or Tris-citrate fructose (P<0.05). Conclusions: Dilution of ram semen with INRA-82 improves semen parameters. Hence, INRA-82, as a stimulating diluent, can be successfully used to reserve viability and sustainability of cooled and cryopreserved ram semen.
文献关键词：
中图分类号：
作者姓名：
Aya M.Fadl;Elshymaa A.Abdelnaby;Hossam R.El-Sherbiny
作者机构：
Theriogenology Department,Faculty of Veterinary Medicine,Cairo University,Egypt
文献出处：
引用格式：
[1]Aya M.Fadl;Elshymaa A.Abdelnaby;Hossam R.El-Sherbiny-.INRA82 extender enhances semen quality in ram under cooled and cryopreserved stages)[J].亚太生殖杂志（英文版）,2022(02):100-104
A类：
INRA82,ram,extenders,Awassi,rams,aliquots,aliquot,INRA,integrities,Thawed
B类：
enhances,semen,quality,under,cooled,cryopreserved,stages,Objective,To,investigate,effect,frozen,conditions,comparison,Tris,select,suitable,Methods,Semen,samples,were,collected,pooled,from,divided,into,three,Each,diluted,one,following,citrate,glucose,fructose,For,liquid,storage,kept,Progressive,motility,measured,after,cooling,Besides,viability,morphology,membrane,acrosome,evaluated,post,cryopreservation,equilibrated,loaded,nitrogen,same,manner,Results,Seminal,characteristics,improved,thawing,compared,those,Conclusions,Dilution,improves,parameters,Hence,stimulating,diluent,can,be,successfully,used,sustainability
AB值：
0.398948
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