典型文献
Aqueous extract of freeze-dried Protaetia brevitarsis larvae promotes osteogenesis by activating β-catenin signaling
文献摘要：
Objective: To investigate the effect of an aqueous extract of Protaetia brevitarsis (AEPB) on osteogenesis using preosteoblast MC3T3-E1 cells and zebrafish larvae. Methods: Flow cytometric analysis was used to measure the cytotoxicy. Alkaline phosphatase activity was detetmined using p-nitrophenyl phosphate as a substrate. Calcium deposition was detected using alizarin red staining along with osteogenic marker expression in preosteoblast MC3T3E1 cells. In addition, vertebral formation in zebrafish larvae was detected using calcein staining and osteogenic gene expression. Results: AEPB highly promoted the expression of osteogenic markers including runt-related transcription factor 2, osterix, and alkaline phosphatase, along with elevated levels of mineralization in MC3T3-E1 cells. Moreover, AEPB accelerated vertebral formation in zebrafish larvae accompanied by upregulated expression of osteogenic genes. FH535, an inhibitor of Wnt/β-catenin, suppressed AEPB-induced osteogenic gene expression and vertebral formation, indicating that AEPB stimulates osteogenesis by activating the Wnt/β-catenin signaling pathway. Conclusions: AEPB stimulates osteoblast differentiation and bone formation by activating β-catenin. Therefore, AEPB is a promising material that induces osteogenesis, and is useful for the treatment of bone resorption diseases.
文献关键词：
中图分类号：
作者姓名：
Jayasingha Arachchige Chathuranga Chanaka Jayasingha;Kyoung Tae Lee;Yung Hyun Choi;Chang-Hee Kang;Mi-Hwa Lee;Gi-Young Kim
作者机构：
Department of Marine Life Science,Jeju National University,Jeju 63243,Republic of Korea;Research Institute for Basic Sciences,Jeju National University,Jeju 63243,Republic of Korea;Forest Biomaterials Research Center,National Institute of Forest Science,Jinju 52817,Republic of Korea;Department of Biochemistry,College of Korean Medicine,Dong-Eui University,Busan 47227,Republic of Korea;Nakdonggang National Institute of Biological Resources,Sangju 37242,Republic of Korea
文献出处：
引用格式：
[1]Jayasingha Arachchige Chathuranga Chanaka Jayasingha;Kyoung Tae Lee;Yung Hyun Choi;Chang-Hee Kang;Mi-Hwa Lee;Gi-Young Kim-.Aqueous extract of freeze-dried Protaetia brevitarsis larvae promotes osteogenesis by activating β-catenin signaling)[J].亚太热带生物医学杂志（英文版）,2022(03):115-123
A类：
AEPB,cytotoxicy,detetmined,MC3T3E1,FH535
B类：
Aqueous,extract,freeze,dried,Protaetia,brevitarsis,larvae,promotes,osteogenesis,by,activating,catenin,signaling,Objective,To,investigate,effect,aqueous,using,preosteoblast,cells,zebrafish,Methods,Flow,cytometric,analysis,was,used,measure,Alkaline,phosphatase,activity,nitrophenyl,phosphate,substrate,Calcium,deposition,detected,alizarin,red,staining,along,osteogenic,expression,In,addition,vertebral,formation,calcein,Results,highly,promoted,markers,including,runt,related,transcription,osterix,alkaline,elevated,levels,mineralization,Moreover,accelerated,accompanied,upregulated,inhibitor,Wnt,suppressed,induced,indicating,that,stimulates,pathway,Conclusions,differentiation,bone,Therefore,promising,material,induces,useful,treatment,resorption,diseases
AB值：
0.472591
相似文献
机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
