Objective:To determine whether pretreatment moxibustion prevents asthma by down-regulating the lung RhoA/ROCK pathway in rats with bronchial asthma and benignly mediating the lung inflammatory response.Methods:Twenty Sprague Dawley(SD)rats were randomly divided into normal control group(C),asthma model group(M),suspended moxibustion 40 min+asthma group(SM40),and suspended moxi-bustion 10 min+asthma group(SM10).Ovalbumin was used as a sensitizer.The two moxibustion groups completed moxibustion treatment lasted 40 min or 10 min respectively 30 min before modeling onset,and was repeated five times in each modeling cycle,for a total of 15 times.Samples were harvested on day 30.Results:Lung impairment was significant in the M group,whereas pretreatment with SM10 and SM40 dramatically attenuated the injury.After modeling,mRNA expression of RhoA and ROCK2 in the lung tissue was significantly higher than that in C group(both P＜0.001),resulting in significant increase in protein levels of IL-17A(P＜0.001).Significant decrease in RhoA and ROCK2 mRNA expression was seen in the SM10(P＜0.001,P＜0.01)and SM40(both P＜0.001)groups compared to that with M rats.The differential trend in the SM40 group was more evident than that in the SM10 group.Regarding IL-10 or IL-17A protein concentration,an upregulation or down-regulation was observed in both SM10(P＜0.05,P＜0.01)and SM40 groups(both P＜0.001)compared to that with the M group.Conclusions:Moxibustion pretreatment significantly prevented pulmonary inflammation in asthmatic rats,potentially via inhibition of the RhoA/ROCK pathway.The efficacty of moxibustion appeared to be significantly associated with the duration of intervention with moxibustion.

郑海珍;邱祺;熊俊;陈骏;管灵聪

江西中医药大学附属医院,江西南昌330009,中国;江西中医药大学研究生院,江西南昌330009,中国

世界针灸杂志（英文版）

[1]郑海珍;邱祺;熊俊;陈骏;管灵聪-.艾灸预处理降低RhoA/ROCK信号表达预防哮喘大鼠肺炎)[J].世界针灸杂志（英文版）,2022(03):230-236

benignly,min+asthma,SM40,moxi,SM10,efficacty

艾灸,RhoA,哮喘,Objective,To,determine,whether,pretreatment,moxibustion,prevents,by,down,regulating,lung,pathway,rats,bronchial,mediating,inflammatory,response,Methods,Twenty,Sprague,Dawley,were,randomly,divided,into,normal,control,suspended,Ovalbumin,was,used,sensitizer,two,groups,completed,lasted,respectively,before,modeling,onset,repeated,five,times,each,cycle,total,Samples,harvested,day,Results,Lung,impairment,whereas,dramatically,attenuated,injury,After,expression,ROCK2,tissue,significantly,higher,than,that,both,resulting,increase,protein,levels,17A,Significant,decrease,seen,compared,differential,trend,more,evident,Regarding,concentration,upregulation,observed,Conclusions,Moxibustion,prevented,pulmonary,inflammation,asthmatic,potentially,via,inhibition,appeared,associated,duration,intervention

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