OBJECTIVE:To investigate the effect of Huangqi decoction(黄芪汤)on renal interstitial fibrosis and its association with the transforming growth factor-β1(TGF-β1)/mitogen-activated protein kinase(MAPK)signaling pathway.METHODS:120 C57/BL mice were randomly divided into six groups:sham group,Enalapril(20 mg/kg)group,5/6 nephrectomy model group,and 5/6 nephrectomy model plus Huangqicoction(0.12,0.36 and 1.08 g/kg respectively)groups.Detecting 24hours urinary protein,blood pressure,serum creatinine,urea nitrogen content changes.Periodic Acid-Schiff stain(PAS)and Masson's trichrome staining was used to observe the renal tissue pathological changes.Protein expression of TGF-p1,Phosphorylated P38 mitogen activated protein kinases(P-P38),Phosphorylated c-jun N-terminal kinase(P-JNK),Phosphorylated extracellular regulated proteinhnase(P-ERK),Fibroblast-specific protein-1(FSP-1),Alpha smooth muscle actin(α-SMA),Type Ⅲ collagen(Collagen Ⅲ),Connective tissue growth factor(CTGF),Bcl-2 Assaciated X protein(Bax)and B cell lymphoma 2(Bcl-2)were measured with western blot and immunohistochemical.RESULTS:Both Huangqi decoction and Enalapril improved the kidney function,24 h urinary protein and the fibrosis in 5/6 nephrectomy mice,Huangqi decoction downregulated the expressions of TGF-β1,FSP-1,α-SMA,Collagen Ⅲ and CTGF in a dose-dependent manner,and it has a significant difference(P＜0.01)compared with model group.Huangqi decoction downregulated the expressions of P-P38,P-JNK,P-ERK and Bcl-2 in a dose-dependent manner,while upregulated the expression of Bax.CONCLUSIONS:The protective effect of Huangqi decoction for renal interstitial fibrosis in 5/6 nep-hrectomized mice via the inhibition of Epithelial-Mesenchymal Transitions and downregulating the TGF-β1/MAPK signaling pathway.

ZHAO Jie;WANG Li;CAO Ai-li;WANG Yun-man;CHI Yang-feng;WANG Yi;WANG Hao;PENG Wen

Department of Chinese Medicine/Department of Chinese Integrative Medicine,The First Affiliated Hospital,Anhui Medical University,Hefei 230022,China;Laboratory of Renal Disease,Putuo Hospital,Shanghai University of Chinese Medicine,Shanghai 200062,China;Department of Nephrology,Putuo Hospital affiliated to Shanghai University of Chinese Medicine,Shanghai 200062,China

中医杂志（英文版）

[1]ZHAO Jie;WANG Li;CAO Ai-li;WANG Yun-man;CHI Yang-feng;WANG Yi;WANG Hao;PENG Wen-.Huangqi decoction(黄芪汤)attenuates renal interstitial fibrosis via transforming growth factor-β1/mitogen-activated protein kinase signaling pathways in 5/6 nephrectomy mice)[J].中医杂志（英文版）,2022(05):723-731

Enalapril,Huangqicoction,24hours,proteinhnase,Assaciated,nep,hrectomized

decoction,黄芪汤,attenuates,renal,interstitial,fibrosis,via,transforming,growth,mitogen,activated,signaling,pathways,nephrectomy,mice,OBJECTIVE,To,investigate,effect,its,association,MAPK,METHODS,C57,BL,were,randomly,divided,into,six,groups,sham,model,plus,respectively,Detecting,urinary,blood,pressure,serum,creatinine,urea,nitrogen,content,changes,Periodic,Acid,Schiff,PAS,Masson,trichrome,staining,was,used,observe,tissue,pathological,Protein,p1,Phosphorylated,P38,kinases,jun,terminal,JNK,extracellular,ERK,Fibroblast,specific,FSP,Alpha,smooth,muscle,actin,SMA,Type,collagen,Collagen,Connective,CTGF,Bcl,Bax,lymphoma,measured,western,blot,immunohistochemical,RESULTS,Both,improved,kidney,function,downregulated,expressions,dose,dependent,manner,has,significant,difference,compared,while,upregulated,CONCLUSIONS,protective,inhibition,Epithelial,Mesenchymal,Transitions,downregulating

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