Gastric cancer is the fifth most frequently occurring and the fourth most lethal malignant cancer worldwide.A bioactive protein(pPeOp)from Omphalia lapidescens exhibits significant inhibitory effects on gastric cancer cells.miRNA deep sequencing analysis shows that miR-30b-5p is significantly upregulated in HGC-27 cells treated with pPeOp.Verification results show that the expression level of miR-30b-5p is significantly increased in HGC-27 cells after pPeOp treatment.Additionally,miR-30b-5p is significantly downregulated in clinical gastric cancer tissues compared to that in adjacent normal tissues.Following pPeOp treatment and/or transfection with miR-30b-5p mimic,the proliferation,migration,and invasion of HGC-27 cells are significantly impaired.Immunofluorescence microscopy shows that pPeOp and/or miR-30b-5p destroy(s)microfilaments and microstructures and inhibit(s)the formation of pseudopodia.Bioinformatics analysis,dual-luciferase reporter assay,and western blot analysis con-firm that miR-30b-5p downregulates Rac1/Cdc42 expression and activation by targeting RAB22A.Available data indicate that miR-30b-5p plays an anti-gastric cancer role in mediating pPeOp.pPeOp upregulates miR-30b-5p expression,which in turn inhibits RAB22A expression,resulting in a reduction in the expression and activation of Rac1 and Cdc42 and their downstream targets,thus destroying the cytoskeletal structure and inhibiting the pro-liferation,migration,and invasion of cancer cells.

Wenjun Xu;Zhenjie Fu;Yuqin Xu;Man Hei Cheung;Yan Chen;Meiai Lin;Hang Wen;Hang Lv;Chun Liang;Jianshu Lou;Yitao Chen

School of Life Science,Zhejiang Chinese Medical University,Hangzhou 310053,China;Division of Life Science,Center for Cancer Research and State Key Lab for Molecular Neural Science,The Hong Kong University of Science and Technology,Hong Kong,China;Department of Gastrointestinal Surgery,the First Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou 310003,China;EnKang Pharmaceuticals(Guangzhou)Ltd.,Guangzhou 510145,China;School of Pharmacy,Hangzhou Normal University,Hangzhou 311121,China

生物化学与生物物理学报（英文版）

[1]Wenjun Xu;Zhenjie Fu;Yuqin Xu;Man Hei Cheung;Yan Chen;Meiai Lin;Hang Wen;Hang Lv;Chun Liang;Jianshu Lou;Yitao Chen-.pPeOp inhibits HGC-27 cell proliferation,migration and invasion by upregulating miR-30b-5p and down-regulating the Rac1/Cdc42 pathway)[J].生物化学与生物物理学报（英文版）,2022(12):1897-1908

pPeOp,Omphalia,lapidescens,microfilaments,pseudopodia,RAB22A

inhibits,HGC,proliferation,migration,invasion,by,upregulating,30b,5p,Rac1,Cdc42,pathway,Gastric,cancer,fifth,most,frequently,occurring,fourth,lethal,malignant,worldwide,bioactive,protein,from,exhibits,inhibitory,effects,gastric,cells,miRNA,deep,sequencing,analysis,shows,that,significantly,upregulated,treated,Verification,results,expression,level,increased,after,treatment,Additionally,downregulated,clinical,tissues,compared,adjacent,normal,Following,transfection,mimic,impaired,Immunofluorescence,microscopy,microstructures,formation,Bioinformatics,dual,luciferase,reporter,assay,western,blot,con,firm,downregulates,activation,targeting,Available,data,indicate,plays,anti,role,mediating,upregulates,which,turn,resulting,reduction,their,downstream,targets,thus,destroying,cytoskeletal,inhibiting

0.428293