Sorbus pohuashanensis(Hance)Hedl.is a potential horticulture and medicinal plant,but its genomic and genetic backgrounds remain unknown.Here,we sequence and assemble the S.pohuashanensis reference genome using PacBio long reads.Based on the new reference genome,we resequence a core collection of 22 Sorbus spp.samples,which are divided into 2 groups(G1 and G2)based on phylogenetic and PCA analyses.These phylogenetic clusters are highly consistent with their classification based on leaf shape.Natural hybridization between the G1 and G2 groups is evidenced by a sample(R21)with a highly het-erozygous genotype.Nucleotide diversity(π)analysis shows that G1 has a higher diversity than G2 and that G2 originated from G1.During the evolution process,the gene families involved in photosynthesis pathways expanded and the gene families involved in energy consumption contracted.RNA-seq data suggests that flavonoid biosynthesis and heat-shock protein(HSP)-heat-shock factor(HSF)pathways play important roles in protection against sunbum.This study provides new insights into the evolution of Sorbus spp.genomes.In addition,the genomic resources,and the identified genetic variations,especially those related to stress resistance,will help future efforts to produce and breed Sorbus spp.

Dongxue Zhao;Yan Zhang;Yizeng Lu;Liqiang Fan;Zhibin Zhang;Jian Zheng;Mao Chai

School of Landscape Architecture,Beijing University of Agriculture,Beijing 102206,China;Shandong Provincial Center of Forest and Grass Germplasm Resources,Jinan,Shandong 250102,China;Institute of Cotton Research of the Chinese Academy of Agricultural Sciences,Anyang,Henan 455000,China

遗传学报

[1]Dongxue Zhao;Yan Zhang;Yizeng Lu;Liqiang Fan;Zhibin Zhang;Jian Zheng;Mao Chai-.Genome sequence and transcriptome of Sorbus pohuashanensis provide insights into population evolution and leaf sunburn response)[J].遗传学报,2022(06):547-558

sunburn,Hedl,horticulture,resequence,erozygous,sunbum

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