典型文献
Gonadal mosaicism mediated female-biased gender control in mice
文献摘要：
Dear Editor,
In nature or during artificial breeding,evolutionary interven-tion or desirable trait selection could be achieved by means of gender control in an organism.Previously reported approaches including sex-sorted semen and genetic manipulation of a certain gene(Holden and Butler,2018;Yosef et al.,2019).In mice,a recent study demonstrated a genetic system for biasing sex ratio through crossing two genetically engineered mouse lines(Yosef et al.,2019).The maternal line encodes functional Cas9 protein on an auto-somal chromosome,whereas the paternal encodes guide RNAs on Y chromosome targeting vital genes.After fertil-ization,males carrying both Cas9 and guide RNAs were self-destructed,resulting in a female biased sex ratio.However,the litter size is proximate half of normal size since half of the progeny are eliminated.Moreover,a reproductive reservoir of males and females must be maintained for crossing.Here,we established a mouse line carrying Hspa2 promoted Cas9 and constitutively expressed sgRNA targeting repetitive sequences on Y chromosome.In those mice,spermatogenic cells carrying Y chromosome were eliminated during sper-matogenesis,resulting in female biased sex ratio in proge-nies whereas the total number was unaffected.
文献关键词：
中图分类号：
作者姓名：
Meizhu Bai;Dan Liang;Yan Cheng;Guolong Liu;Qiudao Wang;Jinsong Li;Yuxuan Wu
作者机构：
State Key Laboratory of Cell Biology,Shanghai Key Laboratory of Molecular Andrology,CAS Center for Excellence in Molecular Cell Science,Shanghai Institute of Biochemistry and Cell Biology,Chinese Academy of Sciences,University of Chinese Academy of Sciences,Shanghai 200031,China;Shanghai Frontiers Science Center of Genome Editing and Cell Therapy,Shanghai Key Laboratory of Regulatory Biology,Institute of Biomedical Sciences and School of Life Sciences,East China Normal University,Shanghai 200241,China;Department of Obstetrics and Gynecology,The First Affiliated Hospital of Anhui Medical University,Hefei 230022,China;NHC Key Laboratory of Study on Abnormal Gametes and Reproductive Tract(Anhui Medical University),Hefei 230032,China;Key Laboratory of Population Health Across Life Cycle(Anhui Medical University),Ministry of Education of the People's Republic of China,Hefei 230032,China
文献出处：
引用格式：
[1]Meizhu Bai;Dan Liang;Yan Cheng;Guolong Liu;Qiudao Wang;Jinsong Li;Yuxuan Wu-.Gonadal mosaicism mediated female-biased gender control in mice)[J].蛋白质与细胞,2022(11):863-868
A类：
Yosef,Hspa2,matogenesis,proge
B类：
Gonadal,mosaicism,mediated,biased,gender,control,mice,Dear,Editor,
In,nature,during,artificial,breeding,evolutionary,interven,desirable,trait,selection,could,achieved,by,means,organism,Previously,reported,approaches,including,sex,sorted,semen,manipulation,certain,Holden,Butler,recent,study,demonstrated,system,biasing,ratio,through,crossing,two,genetically,engineered,mouse,lines,maternal,encodes,functional,Cas9,protein,auto,somal,chromosome,whereas,paternal,guide,RNAs,targeting,vital,After,fertil,ization,carrying,both,were,self,destructed,resulting,However,litter,size,proximate,half,normal,since,progeny,are,eliminated,Moreover,reproductive,reservoir,females,must,maintained,Here,established,promoted,constitutively,expressed,sgRNA,repetitive,sequences,those,spermatogenic,cells,nies,total,number,was,unaffected
AB值：
0.597064
相似文献
机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
