典型文献
Efficient expansion of rare human circulating hematopoietic stem/progenitor cells in steady-state blood using a polypeptide-forming 3D culture
文献摘要：
Although widely applied in treating hematopoietic malignancies,transplantation of hematopoietic stem/progenitor cells(HSPCs)is impeded by HSPC shortage.Whether circulating HSPCs(cHSPCs)in steady-state blood could be used as an alternative source remains largely elusive.Here we develop a three-dimensional culture system(3DCS)including arginine,glycine,aspartate,and a series of factors.Fourteen-day culture of peripheral blood mononuclear cells(PBMNCs)in 3DCS led to 125-and 70-fold increase of the frequency and number of CD34+cells.Further,3DCS-expanded cHSPCs exhibited the similar reconstitution rate com-pared to CD34+HSPCs in bone marrow.Mechanistically,3DCS fabricated an immunomodulatory niche,secreting cytokines as TNF to support cHSPC survival and proliferation.Finally,3DCS could also promote the expansion of cHSPCs in patients who failed in HSPC mobilization.Our 3DCS successfully expands rare cHSPCs,providing an alternative source for the HSPC therapy,particularly for the patients/donors who have failed in HSPC mobilization.
文献关键词：
中图分类号：
作者姓名：
Yulin Xu;Xiangjun Zeng;Mingming Zhang;Binsheng Wang;Xin Guo;Wei Shan;Shuyang Cai;Qian Luo;Honghu Li;Xia Li;Xue Li;Hao Zhang;Limengmeng Wang;Yu Lin;Lizhen Liu;Yanwei Li;Meng Zhang;Xiaohong Yu;Pengxu Qian;He Huang
作者机构：
Bone Marrow Transplantation Center,First Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310012,China;Liangzhu Laboratory,Zhejiang University Medical Center,Hangzhou 311121,China;Institute of Hematology,Zhejiang University,Hangzhou 310012,China;Zhejiang Province Engineering Laboratory for Stem Cell and Immunity Therapy,Hangzhou 310012,China;Core Facilities,Zhejiang University School of Medicine,Hangzhou 310012,China;Dr.Li Dak Sum&Yip Yio Chin Center for Stem Cell and Regenerative Medicine,Zhejiang University,Hangzhou 310012,China
文献出处：
引用格式：
[1]Yulin Xu;Xiangjun Zeng;Mingming Zhang;Binsheng Wang;Xin Guo;Wei Shan;Shuyang Cai;Qian Luo;Honghu Li;Xia Li;Xue Li;Hao Zhang;Limengmeng Wang;Yu Lin;Lizhen Liu;Yanwei Li;Meng Zhang;Xiaohong Yu;Pengxu Qian;He Huang-.Efficient expansion of rare human circulating hematopoietic stem/progenitor cells in steady-state blood using a polypeptide-forming 3D culture)[J].蛋白质与细胞,2022(11):808-824
A类：
cHSPCs,PBMNCs,CD34+cells,CD34+HSPCs,cHSPC
B类：
Efficient,expansion,rare,human,circulating,hematopoietic,progenitor,steady,state,blood,using,polypeptide,forming,culture,Although,widely,applied,treating,malignancies,transplantation,impeded,by,shortage,Whether,could,used,alternative,source,remains,largely,elusive,Here,we,develop,three,dimensional,system,3DCS,including,arginine,glycine,aspartate,series,factors,Fourteen,day,peripheral,mononuclear,fold,increase,frequency,number,Further,expanded,exhibited,similar,reconstitution,rate,com,pared,bone,marrow,Mechanistically,fabricated,immunomodulatory,niche,secreting,cytokines,support,survival,proliferation,Finally,also,promote,patients,who,failed,mobilization,Our,successfully,expands,providing,therapy,particularly,donors,have
AB值：
0.526899
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机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
