典型文献
Epigenetic integrity of paternal imprints enhances the developmental potential of androgenetic haploid embryonic stem cells
文献摘要：
The use of two inhibitors of Mek1/2 and Gsk3β (2i) pro-motes the generation of mouse diploid and haploid embryonic stem cells (ESCs) from the inner cell mass of biparental and uniparental blastocysts,respectively.However,a system enabling long-term maintenance of imprints in ESCs has proven challenging.Here,we report that the use of a two-step a2i (alternative two inhibitors of Src and Gsk3β,TSa2i) derivation/culture protocol results in the establishment of androgenetic haploid ESCs (AG-haESCs) with stable DNA methylation at paternal DMRs (differentially DNA methylated regions)up to passage 60 that can efficiently support generating mice upon oocyte injection.We also show coexistence of H3K9me3 marks and ZFP57 bindings with intact DMR methylations.Furthermore,we demonstrate that TSa2i-treated AG-haESCs are a heterogeneous cell population regarding paternal DMR methylation.Strikingly,AG-haESCs with late passages display increased paternal-DMR methylations and improved developmental poten-tial compared to early-passage cells,in part through the enhanced proliferation of H1g-DMR hypermethylated cells.Together,we establish AG-haESCs that can long-term maintain paternal imprints.
文献关键词：
中图分类号：
作者姓名：
Hongling Zhang;Yuanyuan Li;Yongjian Ma;Chongping Lai;Qian Yu;Guangyong Shi;Jinsong Li
作者机构：
State Key Laboratory of Cell Biology,Shanghai Key Laboratory of Molecular Andrology,CAS Center for Excellence in Molecular Cell Science,Shanghai Institute of Biochemistry and Cell Biology,University of Chinese Academy of Sciences,Chinese Academy of Sciences,Shanghai 200031,China;School of Life Science and Technology,ShanghaiTech University,Shanghai 201210,China;Animal Core Facility,Shanghai Institute of Biochemistry and Cell Biology,Center for Excellence in Molecular Cell Science,Chinese Academy of Sciences,University of Chinese Academy of Sciences,Shanghai 200031,China;School of Life Science,Hangzhou Institute for Advanced Study,University of Chinese Academy of Sciences,Hangzhou 310024,China
文献出处：
引用格式：
[1]Hongling Zhang;Yuanyuan Li;Yongjian Ma;Chongping Lai;Qian Yu;Guangyong Shi;Jinsong Li-.Epigenetic integrity of paternal imprints enhances the developmental potential of androgenetic haploid embryonic stem cells)[J].蛋白质与细胞,2022(02):102-119
A类：
Mek1,blastocysts,a2i,TSa2i,haESCs,bindings,H1g
B类：
Epigenetic,integrity,paternal,imprints,enhances,developmental,potential,androgenetic,haploid,embryonic,cells,two,inhibitors,Gsk3,motes,generation,mouse,diploid,from,inner,mass,biparental,uniparental,respectively,However,system,enabling,long,term,maintenance,has,proven,challenging,Here,report,that,step,alternative,Src,derivation,culture,protocol,results,establishment,AG,stable,DMRs,differentially,regions,can,efficiently,support,generating,mice,upon,oocyte,injection,We,also,show,coexistence,H3K9me3,marks,ZFP57,intact,methylations,Furthermore,demonstrate,treated,heterogeneous,population,regarding,Strikingly,passages,display,increased,improved,compared,early,part,through,enhanced,proliferation,hypermethylated,Together,maintain
AB值：
0.481433
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