Aiming to the enormous requirement for the epidemic defense researches,we designed and constructed a spherical colloidal virus particle(CVP)to mimic nature virus in morphology,physical,chemical and biological characteristics,via coating spiky protein on col-loidal nanoparticles(CNPs)core with bulge hierarchical nanomorphology.The novel virus-like surface nanoparticles can easily be synthesized.The physical,chemical nature and the formation mechanism of the prepared CVPs were characterized and discussed.The synthesized CVPs are similar in size and envelope thickness to common natural viruses.It was demonstrated that the diameter of CVPs is about 238±12 nm,including an 8 nm thickness protein crown with bulges of 33 nm in average width.The CVPs with an isoelectric point of 4.5,meets the native virus property of negative charge under neutral condition.The protein crown enhances the roughness remarkably from 10 nm(CNPs)to 22 nm(CVPs)determined by atomic force microscopy.Thanks to the biomimetic rough morphology,the CVPs show greatly superior cellular uptake performance compared to CNPs,ovalbumin(OVA)and smoothed col-loidal particles(SCPs).The formation mechanism of protein crown with specific thickness can be attributed to the electrostatic in-teraction,protein's flexible structure and specific wettability.These results indicate that the as-prepared artificial virions mimic na-ture viruses in multi-dimension,in terms of size,surface rough morphology,surface negative charge and glycoprotein envelope composition.The synthetic colloidal virus particles pave a facile way toward engineering virus particles substitute for virus-related diseases prevention,diagnostics and cellular delivery vectors.

Hailan Zhong;Hao Wu;Shuoran Chen;Liwei Cao;Changqing Ye;Fan Xia;Fengyu Li

College of Chemistry and Materials Science,Guangdong Provincial Key Laboratory of Functional Supramolecular Coordination Materials and Applications,Guangdong Engineering&Technology Research Centre of Graphene-like Materials and Products,Jinan University,Guangzhou,Guangdong 510632,China;Research Center for Green Printing Nanophotonic Materials,Jiangsu Key Laboratory for Environmental Functional Materials,Suzhou University of Science and Technology,Suzhou,Jiangsu 215009,China;Zhejiang Institute,China University of Geosciences,Hangzhou,Zhejiang 311305,China;School of Materials Science and Engineering,Zhengzhou University,Zhengzhou,Henan 450001,China

中国化学（英文版）

[1]Hailan Zhong;Hao Wu;Shuoran Chen;Liwei Cao;Changqing Ye;Fan Xia;Fengyu Li-.Colloidal Virus Particles with Hierarchical Nanomorphology and Facile Biosurface Modification)[J].中国化学（英文版）,2022(08):946-952

Nanomorphology,Biosurface,loidal,nanomorphology,CVPs,bulges

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