典型文献
Orthogonality of Redesigned tRNA Molecules with Three Stop Codons
文献摘要：
The ability to expand genetic code in living cells has emerged as a powerful method with diverse applications.Here,we design re-placement of the anticodons of E.coli tRNAs that recognize codons for 20 natural amino acids,with three anti-stop codons,resulting in a total of 60 engineered tRNA constructs.We test these constructs one by one in E.coli,and found that six tRNAsCUA(tyrV,serX,hisR,trpT,glnV and leuX),two tRNAsUCA(trpT and leuX)and one tRNAUUA(tyrV)allowed efficient expression of Red Fluorescence Protein(RFP)with the presence of a corresponding stop codon in frame.Furthermore,we exploit the mutual orthogonality of tRNASer CUA,tRNATrpUCA and tRNATyrUUA with corresponding stop codons and demonstrated that the tRNASer CUA and the tRNATrp UCA can provide dynamic range and low crosstalk.Finally,we show the TAG and TGA can not only be used as an"AND gate"circuit to control the translation of target gene,but also be used to control the translation of a prodeoxyviolacein(PDV)pathway and a reporter in parallel.Overall,this work provides flexible tools for translational control and holds great potential to promote synthetic biology studies.
文献关键词：
中图分类号：
作者姓名：
Zhao-Yang Zhang;Dan-Ni Liao;Yu-Xin Ma;Bin Jia;Ying-Jin Yuan
作者机构：
Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(Ministry of Education),School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China;Collaborative Innovation Center of Chemical Science and Engineering(Tianjin),Tianjin University,Tianjin 300072,China
文献出处：
引用格式：
[1]Zhao-Yang Zhang;Dan-Ni Liao;Yu-Xin Ma;Bin Jia;Ying-Jin Yuan-.Orthogonality of Redesigned tRNA Molecules with Three Stop Codons)[J].中国化学（英文版）,2022(07):825-831
A类：
Redesigned,Codons,anticodons,tRNAsCUA,tyrV,serX,hisR,trpT,glnV,leuX,tRNAsUCA,tRNAUUA,tRNATrpUCA,tRNATyrUUA,tRNATrp,prodeoxyviolacein
B类：
Orthogonality,Molecules,Three,Stop,ability,expand,genetic,code,living,cells,has,emerged,powerful,method,diverse,applications,Here,placement,coli,that,recognize,natural,amino,acids,three,stop,resulting,total,engineered,constructs,We,test,these,one,by,found,six,two,allowed,efficient,expression,Fluorescence,Protein,RFP,presence,corresponding,frame,Furthermore,exploit,mutual,orthogonality,tRNASer,demonstrated,can,dynamic,range,crosstalk,Finally,show,TAG,TGA,not,only,be,used,AND,gate,circuit,control,target,but,also,PDV,pathway,reporter,parallel,Overall,this,work,provides,flexible,tools,translational,holds,great,potential,promote,synthetic,biology,studies
AB值：
0.469783
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