Azo dyes are widely applied in the textile industry but are not entirely consumed during the dyeing process and can thus be discharged to the environment in wastewater.However,azo dyes can be degraded using various electron donors,and in this paper,Acid Orange 7(AO7)degradation performance is investigated using methane(CH4)as the sole electron donor.Methane has multiple sources and is readily available and inexpensive.Experiments using 13C-labeled isotopes showed that AO7 degradation was coupled with anaerobic oxidation of methane(AOM)and,subsequently,affected by the initial concentrations of AO7.Higher concentrations of AO7 could inhibit the activity of microorganisms,which was confirmed by the long-term performance of AO7 degradation,with maximum removal rates of 8.94 mg/(L·d)in a batch reactor and 280 mg/(L·d)in a hollow fiber membrane bioreactor(HfMBR).High-throughput sequencing using 16S rRNA genes showed that Candidatus Methanoperedens,affiliated to ANME-2d,dominated the microbial community in the batch reactor and HfMBR.Additionally,the relative abundance of Proteobacteria bacteria(Phenylobacterium,Pseudomonas,and Geothermobacter)improved after AO7 degradation.This outcome suggested that ANME-2d alone,or acting synergistically with partner bacteria,played a key role in the process of AO7 degradation coupled with AOM.

Yanan Bai;Xiuning Wang;Fang Zhang;Raymond Jianxiong Zeng

School of Applied Meteorology,Nanjing University of Information Science and Technology,Nanjing 210044,China;Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation,College of Resources and Environment,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Advanced Laboratory for Environmental Research and Technology,USTC-CityU,Suzhou 215123,China;CAS Key Laboratory of Urban Pollutant Conversion,Department of Applied Chemistry,University of Science and Technology of China,Hefei 230026,China

环境科学与工程前沿

[1]Yanan Bai;Xiuning Wang;Fang Zhang;Raymond Jianxiong Zeng-.Acid Orange 7 degradation using methane as the sole carbon source and electron donor)[J].环境科学与工程前沿,2022(03):77-85

HfMBR,Methanoperedens,Geothermobacter

Acid,Orange,degradation,using,methane,sole,carbon,electron,Azo,dyes,are,widely,applied,textile,industry,but,not,entirely,consumed,during,dyeing,process,can,thus,discharged,environment,wastewater,However,azo,degraded,various,donors,this,paper,AO7,performance,investigated,CH4,Methane,has,multiple,sources,readily,available,inexpensive,Experiments,13C,labeled,isotopes,showed,that,coupled,anaerobic,oxidation,AOM,subsequently,affected,by,initial,concentrations,Higher,could,inhibit,activity,microorganisms,which,confirmed,long,term,maximum,removal,rates,batch,hollow,fiber,membrane,bioreactor,throughput,sequencing,16S,rRNA,genes,Candidatus,affiliated,ANME,2d,dominated,microbial,community,Additionally,relative,abundance,Proteobacteria,Phenylobacterium,Pseudomonas,improved,after,This,outcome,suggested,alone,acting,synergistically,partner,played,key,role

0.571021